采用气相色谱-火焰光度检测法测定小鼠组织中的谷胱甘肽及相关氨基硫醇。

Determination of glutathione and related aminothiols in mouse tissues by gas chromatography with flame photometric detection.

作者信息

Takagi K, Kataoka H, Makita M

机构信息

Faculty of Pharmaceutical Sciences, Okayama University, Japan.

出版信息

Biosci Biotechnol Biochem. 1996 Apr;60(4):729-31. doi: 10.1271/bbb.60.729.

DOI:10.1271/bbb.60.729

PMID:8829550

Abstract

A selective and sensitive method for the determination of glutathione and such related aminothiols as Cys, cysteinylglycine and gamma-glutamylcysteine in mouse tissues by gas chromatography was developed. After fractionating a tissue sample into the free and protein-bound fractions, glutathione and aminothiols in each fraction were converted into their N,S-isopropoxycarbonyl methyl ester derivatives and then measured by gas chromatography with flame photometric detection, using a DB-1 capillary column. This method enabled the redox status of glutathione and related aminothiols in mouse tissues to be investigated.

摘要

建立了一种通过气相色谱法测定小鼠组织中谷胱甘肽以及诸如半胱氨酸、半胱氨酰甘氨酸和γ-谷氨酰半胱氨酸等相关氨基硫醇的选择性灵敏方法。将组织样品分离为游离部分和与蛋白质结合的部分后，将各部分中的谷胱甘肽和氨基硫醇转化为它们的N,S-异丙氧基羰基甲酯衍生物，然后使用DB-1毛细管柱通过带有火焰光度检测的气相色谱法进行测定。该方法能够研究小鼠组织中谷胱甘肽和相关氨基硫醇的氧化还原状态。