Cameron J A, McCaskill C, Kodavanti P R, Wolfe F, Douglas B, Cameron M E, Desaiah D
Department of Biology, Jackson State University, MS 39217, USA.
Int J Vitam Nutr Res. 1995;65(3):215-20.
The correlation between dietary cholesterol, high plasma lipids and cardiovascular disease is well recognized in many species. The purpose of the present study was to examine the effects of high cholesterol and moderately high fat intake of n-3 polyunsaturated fish oil diets on serum lipids in male rats. Male rats were fed either 21% menhaden oil (Control) or 21% menhaden oil with high (2%) cholesterol (MOC) for eight weeks. Whole blood was collected, and analyzed spectrophotometrically for serum cholesterol, triglycerides and lipoproteins. The selected tissues were carefully removed, weighed and analyzed for lipid profiles. The aortas were removed and lipogenesis determined. The results showed that except for spleen the total percent lipid content of heart, lung, liver, adrenal, kidney and brain was not affected in the MOC group. The percent fat content of spleen but not the weight was elevated by 4 fold compared to control. The hematocrit values in the MOC group were unaltered. Serum cholesterol was elevated by 62%, whereas the serum triglycerides and HDL cholesterol were unaltered in MOC group when compared to the MO control. High cholesterol feeding did not affect aortic lipogenesis in the MOC group compared to the control. These results suggest that cholesterol feeding along with n-3 polyunsaturated fish oil diet did not attenuate the anti-atherosclerotic effects of fish oil with the exception of serum cholesterol.
饮食中的胆固醇、高血脂与心血管疾病之间的关联在许多物种中都已得到充分认识。本研究的目的是检验高胆固醇和适度高脂肪摄入的n-3多不饱和鱼油饮食对雄性大鼠血脂的影响。雄性大鼠被喂食21%的鲱鱼油（对照组）或含2%高胆固醇的21%鲱鱼油（MOC组），为期八周。采集全血，用分光光度法分析血清胆固醇、甘油三酯和脂蛋白。仔细取出选定的组织，称重并分析其脂质谱。取出主动脉并测定脂肪生成。结果显示，与对照组相比，除脾脏外，MOC组心脏、肺、肝脏、肾上腺、肾脏和大脑的总脂质含量百分比未受影响。脾脏的脂肪含量百分比而非重量比对照组升高了4倍。MOC组的血细胞比容值未改变。与MO对照组相比，MOC组的血清胆固醇升高了62%，而血清甘油三酯和高密度脂蛋白胆固醇未改变。与对照组相比，高胆固醇喂养并未影响MOC组的主动脉脂肪生成。这些结果表明，除血清胆固醇外，高胆固醇饮食与n-3多不饱和鱼油饮食一起并未减弱鱼油的抗动脉粥样硬化作用。
