Wollin Stephanie D, Wang Yanwen, Kubow Stan, Jones Peter J H
School of Dietetics and Human Nutrition, McGill University, 21,111 Lakeshore Rd, Ste-Anne-de-Bellevue, Quebec, Canada H9X 3V9.
J Nutr Biochem. 2004 Jul;15(7):402-10. doi: 10.1016/j.jnutbio.2003.12.001.
The objective of this study was to examine the effects of the antioxidant alpha-lipoic acid (ALP) versus a medium chain triglyceride oil mixture (MCTo), which was designed to increase energy expenditure and to improve lipid profiles containing medium chain triglycerides, phytosterols, and omega-3 fatty acids in the form of flaxseed oil. A total of 48 hamsters were fed a) hypercholesterolemic (HC) control, b) HC MCTo, c) HC ALP, or d) HC MCTo/ALP diet for 4 weeks. No differences were observed on food intake, body weight, total body water, lean and fat mass, and tissue thiobarbituric acid reactive substances (TBARS). ALP alone had no effect on total cholesterol (TC); however, MCTo feeding increased TC with (P < 0.03) and without (P < 0.003) ALP when compared with control. ALP increased HDL levels compared with control (P < 0.04) and MCTo/ALP (P < 0.007) groups. MCTo, with (P < 0.0001) or without (P < 0.006) ALP, increased non-HDL cholesterol levels versus control. The non-HDL:HDL cholesterol ratio was decreased by ALP compared with MCTo (45%) and MCTo/ALP (68%) (P < 0.0001), a similar trend was seen when compared with the HC control (22%) group (P < 0.14). Triglyceride levels were not altered by any dietary treatment. Liver and heart tissue reduced glutathione (GSH) was increased (P < 0.05) by all three treatments when compared with control. Both tissues showed an increase (P < 0.05) in oxidized glutathione (GSSG) when fed ALP as compared with other treatments. Hamsters fed ALP had a lower (P < 0.05) GSH/GSSG ratio compared with other treatment groups. In conclusion, MCTo feeding does not elicit beneficial effects on circulating plasma lipids and measures of body composition. In addition, our results do not clearly support an improvement in oxidative status through supplementation of ALP. However, our results do support the existence of beneficial effects of ALP on circulating lipoprotein content in the hamster.
本研究的目的是检验抗氧化剂α-硫辛酸（ALP）与中链甘油三酯油混合物（MCTo）的效果，MCTo旨在增加能量消耗并改善含有中链甘油三酯、植物甾醇以及亚麻籽油形式的ω-3脂肪酸的血脂水平。总共48只仓鼠被喂食以下四种饮食4周：a）高胆固醇（HC）对照饮食，b）HC + MCTo饮食，c）HC + ALP饮食，d）HC + MCTo/ALP饮食。在食物摄入量、体重、总体水含量、瘦体重和脂肪量以及组织硫代巴比妥酸反应性物质（TBARS）方面未观察到差异。单独使用ALP对总胆固醇（TC）没有影响；然而，与对照组相比，喂食MCTo时无论是否添加ALP，TC均升高（添加时P < 0.03，未添加时P < 0.003）。与对照组（P < 0.04）和MCTo/ALP组（P < 0.007）相比，ALP可提高高密度脂蛋白（HDL）水平。无论是否添加ALP，MCTo与对照组相比均会升高非HDL胆固醇水平（添加时P < 0.0001，未添加时P < 0.006）。与MCTo（降低45%）和MCTo/ALP（降低68%）相比，ALP可降低非HDL与HDL胆固醇比值（P < 0.0001）；与HC对照组（降低22%）相比也观察到类似趋势（P < 0.14）。任何饮食处理均未改变甘油三酯水平。与对照组相比，所有三种处理均使肝脏和心脏组织中的还原型谷胱甘肽（GSH）增加（P < 0.05）。与其他处理相比，喂食ALP时，两种组织中的氧化型谷胱甘肽（GSSG）均增加（P < 0.05）。与其他处理组相比，喂食ALP的仓鼠GSH/GSSG比值更低（P < 0.05）。总之，喂食MCTo对循环血浆脂质和身体成分指标没有有益影响。此外，我们的结果并未明确支持通过补充ALP改善氧化状态。然而，我们的结果确实支持ALP对仓鼠循环脂蛋白含量存在有益影响。
