Faix J, Dittrich W
Max-Planck-Institut für Biochemie, Martinsried, Germany.
FEBS Lett. 1996 Oct 7;394(3):251-7. doi: 10.1016/0014-5793(96)00963-5.
A protein accumulated in the cortical region of Dictyostelium discoideum cells proved to be a homologue of GTPase activating proteins that are responsible for the inactivation of ras in yeast and man. Elimination of this protein, DGAP1, by gene replacement resulted in an increased rate of growth of D. discoideum cells on bacterial lawns, and in the formation of aberrant, multi-tipped fruiting bodies. Overexpression of DGAP1 caused the cells to become multi-nucleated since chromosome segregation during mitosis was not reliably followed by cleavage of the cells. These results suggest that in D. discoideum, ras or a related small GTP-binding protein is involved in regulating growth based on the phagocytosis of bacteria, and in coupling activities of the cell cortex to the organization of spindle and asters in mitotic cells.
在盘基网柄菌细胞的皮质区域积累的一种蛋白质被证明是GTP酶激活蛋白的同源物，这种蛋白负责酵母和人类中ras的失活。通过基因替换消除这种蛋白质DGAP1，导致盘基网柄菌细胞在细菌菌苔上的生长速率增加，并形成异常的、多尖端的子实体。DGAP1的过表达导致细胞多核化，因为有丝分裂期间的染色体分离并没有可靠地伴随着细胞分裂。这些结果表明，在盘基网柄菌中，ras或相关的小GTP结合蛋白参与基于细菌吞噬作用调节生长，并参与将细胞皮质的活动与有丝分裂细胞中纺锤体和星体的组织相耦合。
