Une M, Harada J, Mikami T, Hoshita T
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.
J Chromatogr B Biomed Appl. 1996 Jun 28;682(1):157-61. doi: 10.1016/0378-4347(96)00107-7.
This paper describes the high-performance liquid chromatographic separation of UV-absorbing bile alcohol derivatives. Bile alcohols were treated with 3 alpha-hydroxysteroid dehydrogenase to form the corresponding 3-keto bile alcohols. The 3-keto bile alcohols produced were converted to the 2,4-dinitrophenylhydrazone derivatives, separated using a Nova-Pak Phenyl column, and monitored at 364 nm. The separation of stereoisomers related to the configuration of hydroxyl groups on the side chain of the bile alcohols, which was not achieved by gas chromatography, could also be accomplished.
本文描述了紫外吸收胆汁醇衍生物的高效液相色谱分离。胆汁醇用3α-羟基类固醇脱氢酶处理以形成相应的3-酮胆汁醇。产生的3-酮胆汁醇转化为2,4-二硝基苯腙衍生物，使用Nova-Pak苯基柱进行分离，并在364nm处进行监测。胆汁醇侧链上羟基构型相关的立体异构体的分离，这是气相色谱无法实现的，也能够完成。
