Fayein N A, Head M W, Jeanny J C, Courtois Y, Fuhrmann G
Unite de Recherches Gerontologiques, INSERM, Developpement et Senescence Cellulaire, Paris, France.
J Neurosci Res. 1996 Mar 1;43(5):602-12. doi: 10.1002/(SICI)1097-4547(19960301)43:5<602::AID-JNR10>3.0.CO;2-2.
The expression of the chicken cysteine-rich fibroblast growth factor receptor (CFR) during organogenesis and specifically during retina formation was studied by Northern blotting and a sensitive in situ hybridization. At days 2 and 4 of embryonic development (E2 and E4), CFR mRNA was present in a wide variety of developing organs; it was abundantly expressed in nervous structures, particularly in the retina. The levels of CFR transcripts were high during the proliferation and the subsequent differentiation phases of retinal neurogenesis, reached a maximum around E11 during the onset of the major period of retinal cell death, and then declined progressively. CFR mRNA was not detected at late stages when the final arrangement of retinal cell layers has been established. In prolonged primary cell cultures of chicken embryo retina, CFR expression showed a similar down-regulation to that seen with increasing age in vivo. It was up-regulated either directly or indirectly by its ligands. The CFR expression pattern in the developing retina was complementary to that of two other fibroblast growth factor (FGF) receptors, namely FGF-R1 and FGF-R2. In regard to a progressive increase in the expression of their ligands during retinal development, we suggest that CFR may have a role distinct from that of the tyrosine kinase FGF receptors during retinogenesis. Finally, the comparison of CFR expression with those of the other high affinity receptors indicates a regulation of the FGF function at the receptor level during neural retina development.
通过Northern印迹法和灵敏的原位杂交技术，研究了富含半胱氨酸的鸡成纤维细胞生长因子受体（CFR）在器官发生过程中，特别是在视网膜形成过程中的表达情况。在胚胎发育的第2天和第4天（E2和E4），CFR mRNA存在于多种发育中的器官中；它在神经结构中大量表达，尤其是在视网膜中。在视网膜神经发生的增殖和随后的分化阶段，CFR转录本水平很高，在视网膜细胞死亡的主要时期开始时，即E11左右达到最高，然后逐渐下降。在视网膜细胞层最终排列形成的后期阶段未检测到CFR mRNA。在鸡胚视网膜的长期原代细胞培养中，CFR表达显示出与体内随年龄增长所见相似的下调。它被其配体直接或间接上调。发育中的视网膜中CFR的表达模式与另外两种成纤维细胞生长因子（FGF）受体，即FGF-R1和FGF-R2的表达模式互补。关于它们的配体在视网膜发育过程中的表达逐渐增加，我们认为CFR在视网膜发生过程中可能具有与酪氨酸激酶FGF受体不同的作用。最后，将CFR表达与其他高亲和力受体的表达进行比较表明，在神经视网膜发育过程中，FGF功能在受体水平受到调控。
