Koivusalo A, Makisalo H, Talja M, Cormio L, Ruutu M, Wolff H, Hockerstedt K
Fourth Department of Surgery, Helsinki University Central Hospital, Finland.
Res Exp Med (Berl). 1996;196(1):53-66. doi: 10.1007/BF02576828.
Toxicity of materials used in indwelling drains or catheters has the potential to damage surrounding tissues. The biocompatibility of biliary T-tubes was investigated using in groups of piglets (total 30 animals). In group 1 (n=16) a choledochotomy was performed with insertion of a latex T-tube (n=6) or a silicone T-tube (n=8), or without a T-tube (n=2). In group 2 (n=14) the common bile duct (CBD) was 3/4-transected, and the lesion was sutured over a latex T-tube (n=4) or a silicone T-tube (n=5), or without a T-tube (n=5). The groups were reoperated upon after 2 and 6 weeks respectively, and the CBD was harvested for scanning electron microscopy and light microscopy. The T-tubes were examined for cell culture toxicity with a DNA synthesis inhibition test. According to the cell culture tests, latex T-tubes were toxic and silicone T-tubes nontoxic. T-tubes induced moderate to pronounced fibrosis and epithelial damage in the CBD wall, but did not induce excessive fibrosis or scarring at the site of CBD suture. In the 6-week study period, however, the grade of tissue reactions in the CBD did not correlate with the toxicity of the T-tube materials, but rather reflected a foreign body reaction and mechanical pressure. Although gross anatomical changes did not occur, neither material seemed to be completely harmless for porcine CBD wall.
留置引流管或导管所用材料的毒性有可能损害周围组织。使用仔猪组（共30只动物）研究了胆管T形管的生物相容性。在第1组（n = 16）中，进行胆总管切开术，插入乳胶T形管（n = 6）或硅胶T形管（n = 8），或不插入T形管（n = 2）。在第2组（n = 14）中，胆总管（CBD）被3/4横断，病变在乳胶T形管（n = 4）或硅胶T形管（n = 5）上缝合，或不使用T形管（n = 5）。分别在2周和6周后对各组进行再次手术，采集胆总管用于扫描电子显微镜和光学显微镜检查。通过DNA合成抑制试验检测T形管的细胞培养毒性。根据细胞培养试验，乳胶T形管有毒，硅胶T形管无毒。T形管在胆总管壁诱导中度至明显的纤维化和上皮损伤，但在胆总管缝合部位未诱导过度纤维化或瘢痕形成。然而，在6周的研究期内，胆总管中的组织反应程度与T形管材料的毒性无关，而是反映了异物反应和机械压力。虽然未发生大体解剖学变化，但两种材料似乎对猪胆总管壁都不完全无害。
