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[脆弱拟杆菌产肠毒素菌株的选定特性]

[Selected properties of Bacteroides fragilis enterotoxigenic strains].

作者信息

Rokosz A, Meisel-Mikołajczyk F

机构信息

Zakład Bakteriologii Klinicznej AM w Warszawie.

出版信息

Med Dosw Mikrobiol. 1995;47(3-4):183-8.

PMID:8833930
Abstract

Seven B. fragilis strains were examined. One strain was reference, non-enterotoxigenic, representing serotype E2 according to Beerens et al. (1971). Six strains produced enterotoxin (ETBF). Four of them were isolated from human feces and two of them from swine feces. All strains were investigated morphologically, biochemically (ATB Expression, France) and by means of direct immunofluorescence (Bacteroides--IF test, Poland). Their resistance to chemotherapeutics was tested (ATB Expression, France). The MIC values of clindamycin and metronidazole were determined using E test strips (AB Biodisk, Sweden). Each strain formed capsules. The percentage of encapsulated cells was high (80-100%). Some strains produced thick capsules. Biochemical patterns were similar and typical for B. fragilis rods. One enterotoxigenic strain produced gelatinase and three ETBF strains fermented trehalose. All strains reacted in direct immunofluorescence exclusively with conjugate against serotype E of BFG. Thus, each strain showed antigenic pattern E. Drug susceptibility of all strains was similar. One enterotoxigenic strain was resistant to clindamycin. All strains were susceptible to metronidazole. These studies indicate that on the basis of morphological, biochemical and serological features (IF), enterotoxigenic B. fragilis strains cannot be distinguished from the nonenterotoxigenic one. Also, the correlation between toxigenicity and drug sensitivity of the examined strains is not observed.

摘要

对7株脆弱拟杆菌菌株进行了检测。1株为参考菌株,不产肠毒素,根据Beerens等人(1971年)的分类代表E2血清型。6株产生肠毒素(ETBF)。其中4株从人类粪便中分离得到,2株从猪粪便中分离得到。所有菌株均进行了形态学、生化检测(法国梅里埃ATB Expression系统)以及直接免疫荧光检测(波兰的拟杆菌免疫荧光试验)。检测了它们对化疗药物的耐药性(法国梅里埃ATB Expression系统)。使用E试验条(瑞典AB Biodisk公司)测定了克林霉素和甲硝唑的最低抑菌浓度(MIC)值。每株菌都形成荚膜。荚膜包裹细胞的比例很高(80%-100%)。一些菌株产生厚荚膜。生化模式与脆弱拟杆菌杆菌相似且典型。1株产肠毒素菌株产生明胶酶,3株ETBF菌株发酵海藻糖。所有菌株在直接免疫荧光检测中仅与针对脆弱拟杆菌E血清型的结合物发生反应。因此,每株菌都呈现抗原模式E。所有菌株的药敏性相似。1株产肠毒素菌株对克林霉素耐药。所有菌株对甲硝唑敏感。这些研究表明,基于形态学、生化和血清学特征(免疫荧光),产肠毒素的脆弱拟杆菌菌株与不产肠毒素的菌株无法区分。此外,在所检测菌株中未观察到产毒性与药物敏感性之间的相关性。

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