Meera G, Jo H, Ravi R
R&D Histopath Lab, Madras, India.
Eur J Morphol. 1995 Nov;33(4):337-58.
MiB-1 antibody staining can be carried out on formalin fixed, paraffin embedded tissue only if microwave antigen retrieval is performed. This method was used to identify proliferating cells in archival paraffin sections of 137 cases of human brain tumors. The tumors concerned were astrocytomas (57), glioblastomas (16), oligodendrogliomas (11), mixed gliomas (8), ependymomas (9), subependymoma (2), medulloblastomas (4) and meningiomas (30). For the tumors which were histologically graded (the astrocytomas, oligodendrogliomas and ependymomas), generally the scores of MiB-1 staining were correlated with the tumor grade, although there were exceptions to this rule, for example, a few low grade tumors displayed high MiB-1 counts while some highly malignant brain tumors failed to display any positive staining for MiB-1, despite having high mitotic counts. This phenomenon was probably due to loss of proliferating antigens. In the MiB-1 positive sections, hot spots with a high mitotic activity could be identified with ease and the abnormal morphology of the tumor nuclei and of the atypical mitoses were highlighted by the contrasting intensily brown staining. In addition, the dividing cell type could be easily identified and differences in mitotic behavior of invasive and necrotic parts of tumors could be observed.
只有在进行微波抗原修复的情况下，才能对福尔马林固定、石蜡包埋的组织进行MiB-1抗体染色。该方法用于鉴定137例人脑肿瘤存档石蜡切片中的增殖细胞。所涉及的肿瘤包括星形细胞瘤（57例）、胶质母细胞瘤（16例）、少突胶质细胞瘤（11例）、混合性胶质瘤（8例）、室管膜瘤（9例）、室管膜下瘤（2例）、髓母细胞瘤（4例）和脑膜瘤（30例）。对于组织学分级的肿瘤（星形细胞瘤、少突胶质细胞瘤和室管膜瘤），一般来说，MiB-1染色的分数与肿瘤分级相关，尽管也有例外情况，例如，一些低级别肿瘤显示出高MiB-1计数，而一些高度恶性的脑肿瘤尽管有高有丝分裂计数，但MiB-1却未显示任何阳性染色。这种现象可能是由于增殖抗原的丢失。在MiB-1阳性切片中，可以轻松识别出有高有丝分裂活性的热点，并且通过强烈的棕色染色对比突出了肿瘤细胞核和非典型有丝分裂的异常形态。此外，可以轻松识别分裂细胞类型，并观察到肿瘤侵袭和坏死部分有丝分裂行为的差异。
