Kidogawa Hideo, Nanashima Atsushi, Yano Hiroshi, Matsumoto Megumi, Yasutake Toru, Nagayasu Takeshi
Division of Surgical Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Anticancer Res. 2005 Nov-Dec;25(6B):3957-62.
Argyrophilic nucleolar organizer regions (AgNORs) and MIB-1 as proliferating activities have been applied separately to assess the malignant potential of cancer cells. We conducted staining of AgNORs and MIB-1 in 42 surgically-resected invasive breast carcinomas.
Paraffin-embedded sections were used for double staining and the mean AgNOR counts in 100 MIB-1-positive and -negative cells were calculated.
The mean AgNOR count in MIB-1-positive cells was significantly higher than in MIB-1-negative ones. AgNOR counts in MIB-1-positive tumors were significantly higher in tumors > or =2 cm and those with positive nodes. Multivariate analysis identified the AgNOR count in MIB-1-positive tumors as the only independent factor related to node metastasis. Survival of patients with lower counts of AgNORs in MIB-1-positive tumors was significantly better compared to those with higher counts.
Double staining of MIB-1 and AgNORs is useful for predicting lymph node metastasis and prognosis of patients with breast carcinoma.
嗜银核仁组织区（AgNORs）和MIB-1作为增殖活性指标已分别用于评估癌细胞的恶性潜能。我们对42例手术切除的浸润性乳腺癌进行了AgNORs和MIB-1染色。
采用石蜡包埋切片进行双重染色，并计算100个MIB-1阳性和阴性细胞中的平均AgNOR计数。
MIB-1阳性细胞中的平均AgNOR计数显著高于MIB-1阴性细胞。在肿瘤直径≥2 cm和有阳性淋巴结的MIB-1阳性肿瘤中，AgNOR计数显著更高。多因素分析确定MIB-1阳性肿瘤中的AgNOR计数是与淋巴结转移相关的唯一独立因素。MIB-1阳性肿瘤中AgNOR计数较低的患者生存率明显高于计数较高的患者。
MIB-1和AgNORs双重染色有助于预测乳腺癌患者的淋巴结转移和预后。
