Ho W Z, Cnaan A, Li Y H, Zhao H, Lee H R, Song L, Douglas S D
Department of Pediatrics, University of Pennsylvania Medical School, Philadelphia 19104, USA.
AIDS Res Hum Retroviruses. 1996 Feb 10;12(3):195-8. doi: 10.1089/aid.1996.12.195.
Substance P (SP), a member of the tachykinin family of neuropeptides, is an important immunomodulator of lymphocyte and monocyte/macrophage function. We have examined the effects of SP on human immunodeficiency virus type 1 (HIV-1) infection of peripheral blood monocyte-derived macrophages (MDMs) in vitro. Human monocytes isolated by Ficoll gradient followed by adherence were maintained in vitro for 10 days and infected with HIV-1. The addition of SP resulted in a 2- to 8-fold-enhanced HIV-1 expression in the MDMs isolated from 7 of 13 healthy donors as determined by reverse transcriptase (RT) activity and p24 protein expression assays, as compared to control cultures incubated with HIV-1 alone. There was no correlation observed, however, between SP-stimulated TNF production and HIV-1 expression in MDMs obtained from a subset of these donors. These effects of SP on HIV-1 expression in MDMs in vitro may have in vivo implications relevant to modulation of monocyte/macrophage functions, to HIV-1 infection of monocytes/macrophages, and to the immunopathogenesis of HIV-1 infection.
P物质（SP）是神经肽速激肽家族的一员，是淋巴细胞和单核细胞/巨噬细胞功能的重要免疫调节因子。我们在体外研究了SP对人外周血单核细胞来源的巨噬细胞（MDM）感染1型人类免疫缺陷病毒（HIV-1）的影响。通过Ficoll梯度离心分离并经贴壁处理的人单核细胞在体外培养10天，然后感染HIV-1。与仅用HIV-1培养的对照培养物相比，添加SP后，通过逆转录酶（RT）活性和p24蛋白表达测定发现，从13名健康供体中的7名分离出的MDM中HIV-1表达增强了2至8倍。然而，在从这些供体的一个亚组获得的MDM中，未观察到SP刺激的TNF产生与HIV-1表达之间存在相关性。SP对体外MDM中HIV-1表达的这些影响可能在体内与单核细胞/巨噬细胞功能的调节、单核细胞/巨噬细胞的HIV-1感染以及HIV-1感染的免疫发病机制相关。
