Brown D R, Rawlings K, Handy V, Fife K H, Bryan J T, Cramer H, Graham M
Department of Medicine, Richard L. Roudebush Veterans Administration Medical Center, Indianapolis, Indiana, USA.
J Med Virol. 1996 Feb;48(2):210-4. doi: 10.1002/(SICI)1096-9071(199602)48:2<210::AID-JMV15>3.0.CO;2-I.
Infection of the uterine cervix with human papillomavirus (HPV) is associated with dysplastic lesions that may progress to malignancy. Certain HPV types are associated with higher risk of cervical cancer than other genital HPVs. The goal of this study was to determine if cells obtained by cervicovaginal lavage contain similar HPV types as paired cervical biopsy in women referred because of abnormal cervical cytology. Thirty-four paired lavage and biopsy samples were analyzed for HPV DNA by hybrid capture, using "low risk" (HPV types 6. 11, and related types and "high risk" group (HPV types 16, 18, and related types) HPV. HPV was detected in 24 lavage samples and 18 biopsies. High risk types were predominant. In 14 of 18 HPV-positive biopsies, the paired lavage was also positive for the same HPV group. Four biopsies were HPV-positive at low levels, and the paired lavage was HPV-negative. The mean viral copy numbers of the biopsies from patients with positive and negative lavage samples were 2.7 and 0.1, respectively (P = .02). Ten low level HPV infections were detected by lavage that were not detected by biopsy. HPV detection by hybrid capture in cells obtained by cervicovaginal lavage reflects the results of HPV testing in cervical biopsies.
人乳头瘤病毒（HPV）感染子宫颈与发育异常病变相关，这些病变可能会发展为恶性肿瘤。某些HPV类型比其他生殖器HPV类型导致宫颈癌的风险更高。本研究的目的是确定因宫颈细胞学异常而转诊的女性，通过宫颈阴道灌洗获得的细胞是否含有与配对宫颈活检相似的HPV类型。使用“低风险”（HPV 6型、11型及相关类型）和“高风险”组（HPV 16型、18型及相关类型）HPV，通过杂交捕获法对34对灌洗和活检样本进行HPV DNA分析。在24份灌洗样本和18份活检样本中检测到HPV。高风险类型占主导。在18份HPV阳性活检样本中的14份中，配对的灌洗样本对同一HPV组也呈阳性。4份活检样本HPV低水平阳性，而配对的灌洗样本HPV阴性。灌洗样本阳性和阴性患者活检样本的平均病毒拷贝数分别为2.7和0.1（P = 0.02）。通过灌洗检测到10例活检未检测到的低水平HPV感染。通过杂交捕获法在宫颈阴道灌洗获得的细胞中检测HPV，反映了宫颈活检中HPV检测的结果。
