Kanga U, Mehra N K, Larrea C L, Lardy N M, Kumar A, Feltkamp T E
Department of Histocompatibility and Immunogenetics, All India Institute of Medical Sciences, New Delhi.
Clin Rheumatol. 1996 Jan;15 Suppl 1:13-8. doi: 10.1007/BF03342638.
In this study, 60 HLA-B27+ve SSA patients and 17 healthy controls belonging to North India were analyzed to ascertain heterogeneity of the B27 molecule in this population. ID-IEF and PCR-SSOP technologies were used to analyze polymorphism in exon 2 and 3 of the HLA-B27 gene. Four different subtypes were encountered: B2702,04,05 and 07. Other subtypes of B27 viz B2701,03,06 and 08 were not encountered. B2704 (common oriental subtype) and B2705 (common Caucasian subtype) were the most common subtypes in the control and patient groups. B2707 was less frequently encountered in both groups and B2702 was found in only one AAU patient. B2704 was the predominant subtype in the AS group (70.8%) compared to its frequency of 47% in healthy controls (RR = 2.73) while in the undiff SpA group, B2705 occurred most frequently (73.1%, RR = 3.05). B27 subtypes segregated differently in males and females. 12 of the 17 male AS patients carried B2704 as compared to 1 of 8 healthy males (X2 = 3.9, P < 0.05). On the other hand, in the undiff SpA, B2705 was significantly raised in female patients (100%) as compared to healthy females (22.2%, X2 = 4.9, P < 0.05). Subtype distribution is indicative of racial admixture in the Asian Indian population.
在本研究中，对60名来自印度北部的HLA - B27阳性的葡萄膜炎性关节炎（SSA）患者和17名健康对照者进行分析，以确定该人群中B27分子的异质性。采用等电聚焦免疫固定电泳（ID - IEF）和聚合酶链反应 - 序列特异性寡核苷酸探针杂交（PCR - SSOP）技术分析HLA - B27基因第2和第3外显子的多态性。共发现四种不同的亚型：B2702、04、05和07。未发现B27的其他亚型，即B2701、03、06和08。B2704（常见的东方亚型）和B2705（常见的白种人亚型）是对照组和患者组中最常见的亚型。B2707在两组中出现的频率较低，仅在1例急性前葡萄膜炎（AAU）患者中发现B2702。与健康对照者中47%的频率相比，B2704是强直性脊柱炎（AS）组中的主要亚型（70.8%，相对危险度RR = 2.73）；而在未分化脊柱关节炎（undiff SpA）组中，B2705出现的频率最高（73.1%，RR = 3.05）。B27亚型在男性和女性中的分布有所不同。17名男性AS患者中有12名携带B2704，而8名健康男性中只有1名携带（X2 = 3.9，P < 0.05）。另一方面，在未分化脊柱关节炎中，女性患者中B2705的比例显著升高（100%），而健康女性中该比例为22.2%（X2 = 4.9，P < 0.05）。亚型分布表明亚洲印度人群中存在种族混合现象。
