Takaya K, Yoshimasa T, Arai H, Tamura N, Miyamoto Y, Itoh H, Nakao K
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine.
Intern Med. 1996 Jun;35(6):449-52. doi: 10.2169/internalmedicine.35.449.
In order to evaluate the role of RET proto-oncogene in the development and growth of pheochromocytomas, we examined mutations in RET and expression of RET in 7 cases of sporadic pheochromocytomas. Tumors were screened for mutations in exons 10 and 11 and codon 918 which are identified in multiple endocrine neoplasia types 2A and 2B. No mutations were found in these regions in all of the sporadic pheochromocytomas examined. On the other hand, RET mRNA was detected in all pheochromocytomas and the levels of RET expression were higher in 5 of 7 pheochromocytomas than in normal adrenal medulla, indicating that RET is overexpressed in a sizable portion of sporadic pheochromocytomas. These results suggest that high levels of expression of RET may have relevance to the development or growth of sporadic pheochromocytomas.
为了评估RET原癌基因在嗜铬细胞瘤发生和生长中的作用，我们检测了7例散发性嗜铬细胞瘤中RET的突变情况及RET的表达。对肿瘤进行外显子10和11以及密码子918的突变筛查，这些区域的突变在2A和2B型多发性内分泌肿瘤中已得到确认。在所检测的所有散发性嗜铬细胞瘤中，这些区域均未发现突变。另一方面，在所有嗜铬细胞瘤中均检测到RET mRNA，7例嗜铬细胞瘤中有5例的RET表达水平高于正常肾上腺髓质，这表明在相当一部分散发性嗜铬细胞瘤中RET呈过表达。这些结果提示，RET的高表达水平可能与散发性嗜铬细胞瘤的发生或生长有关。
