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A novel screening system based on gene targeting to enrich the modified mammalian cells: without leaving selection marker and additional sequence.
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Site-directed point mutations in embryonic stem cells: a gene-targeting tag-and-exchange strategy.
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Double replacement: strategy for efficient introduction of subtle mutations into the murine Col1a-1 gene by homologous recombination in embryonic stem cells.
Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2819-23. doi: 10.1073/pnas.91.7.2819.
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Immunotoxin-mediated conditional disruption of specific neurons in transgenic mice.
Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):1132-6. doi: 10.1073/pnas.92.4.1132.
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Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter.
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Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes.
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A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin.
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Homologous recombination at c-fyn locus of mouse embryonic stem cells with use of diphtheria toxin A-fragment gene in negative selection.
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Anti-Tac(Fv)-PE40, a single chain antibody Pseudomonas fusion protein directed at interleukin 2 receptor bearing cells.
J Biol Chem. 1990 Sep 5;265(25):15198-202.
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Infertility in male transgenic mice: disruption of sperm development by HSV-tk expression in postmeiotic germ cells.
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