Jha L J, Santos J D, Knowles J C
INEB, National Institute of Biomedical Engineering, University of Oporto, Porto, Portugal.
J Biomed Mater Res. 1996 Aug;31(4):481-6. doi: 10.1002/(SICI)1097-4636(199608)31:4<481::AID-JBM7>3.0.CO;2-H.
P2O5-based glass hydroxyapatite (HA) composites denoted HA-2 oxide, HA-3 oxide, and HA-4-oxide, were immersed in Hank's balanced salt solution for a period of 1, 2, 3, and 4 weeks at 37 degrees C in nonagitated condition for in vitro evaluation. A surface layer was precipitated on the composites that was analyzed using scanning electron microscopy (SEM), X-ray photoelecton spectroscopy, and thin film X-ray diffractometry (TF-XRD). SEM micrographs showed complete coverage of the composite surface by crystallites after immersion of 1 week in the solution, which grew thicker with respect to immersion time. The binding energies measured by XPS indicated apatite formation and the presence of carbonate on the composite surface, showing the newly formed layer was a carbonated apatite. Confirmation of the formed apatite layer was obtained by TF-XRD.
基于五氧化二磷的玻璃羟基磷灰石（HA）复合材料，分别标记为HA-2氧化物、HA-3氧化物和HA-4氧化物，在37℃的非搅拌条件下于汉克平衡盐溶液中浸泡1、2、3和4周，用于体外评估。在复合材料上沉淀出一层表面层，使用扫描电子显微镜（SEM）、X射线光电子能谱和薄膜X射线衍射仪（TF-XRD）对其进行分析。SEM显微照片显示，在溶液中浸泡1周后，复合材料表面被微晶完全覆盖，随着浸泡时间的延长，微晶层变得更厚。通过XPS测量的结合能表明在复合材料表面形成了磷灰石且存在碳酸盐，这表明新形成的层是碳酸化磷灰石。通过TF-XRD证实了形成的磷灰石层。
