Scholtens R G, Adams S R
Am J Vet Res. 1977 Jul;38(7):1099-101.
Between November, 1974, and March, 1976, skin biopsies were taken at 12 different times from a small herd of 8 to 10 cattle near Lawrenceville, Ga. Samples were incubated for 4 to 6 hours in Hanks' balanced salt solution and centrifuged, and the sediment was examined microscopically for microfilariae. Microfilariae were consistently found in 4 of 10 animals over a 12-month period; numbers of microfilariae ranged from 1 to 211, with a mean of 3.42, 12.89, 17.31, and 27.53 per 4-mm-diameter biopsy from the 4 individual animals. The parasite was evidently being transmitted on the farm, as evidenced by the presence of microfilariae in an animal born on the farm.
1974年11月至1976年3月期间，在佐治亚州劳伦斯维尔附近一小群8至10头牛中，于12个不同时间采集了皮肤活检样本。样本在汉克斯平衡盐溶液中孵育4至6小时后离心，沉淀物经显微镜检查寻找微丝蚴。在12个月的时间里，10头牛中有4头持续发现有微丝蚴；4头个体动物每4毫米直径活检样本中的微丝蚴数量在1至211条之间，平均分别为3.42条、12.89条、17.31条和27.53条。农场中一头出生的动物体内存在微丝蚴，这表明该寄生虫显然在农场中传播。
