Traub W H, Eiden A, Leonhard B, Bauer D
Institut für Medizinische Mikrobiologie und Hygiene, Universität des Saarlandes, Homburg/Saar, Germany.
Zentralbl Bakteriol. 1996 Jun;284(1):124-35. doi: 10.1016/s0934-8840(96)80161-7.
A total of 129 selected isolates of Serratia marcescens which had been recovered from 50 patients during the 1980-1995 period and which revealed phenotypic variation in terms of bacteriocin (phage tail) susceptibility, carbon source assimilation, or serotype, were reexamined with these three phenotypic methods. Seven isolates (5.4%) were bacteriocin nontypable; all 129 isolates utilized carbon sources and could be serotyped. Fourty-eight isolates from 20 patients yielded unambiguous results with these 3 phenotypic methods and were excluded from further analysis. Among the remaining 81 isolates from 30 patients, isolates from 2 patients revealed phenotypic variation in bacteriocin susceptibility only, whereas isolates from 6 patients showed variant bacteriocin types and variant biochemical profiles, but were of identical serotype. Isolates from 20 patients revealed variant biochemical profiles only. Three patients had become superinfected with strains of S. marcescens of different phenotype and genotype. In 4 patients, previously motile (H12) isolates had become nonmotile (H-). PFGE analysis of XbaI and SpeI-restricted genomic DNA of the 81 isolates of the 30 patients demonstrated the isolates of 22 patients to be genotypically identical. The isolates from 3 patients were closely related by genotype, and those from an additional patient proved to be possibly related. PFGE analysis demonstrated one patient to have become infected by two genotypically different strains of S. marcescens of identical serotype, which, however, differed in bacteriocin type and biochemical profile. It was concluded that PFGE analysis of restricted genomic S. marcescens DNA was superior to the three phenotypic methods examined comparatively. Serotyping was more reliable than bacteriocin typing, and the latter technique yielded fewer phenotypic variants than determination of biochemical profiles among consecutively recovered isolates from patients with long-lasting S. marcescens infection.
共选取了129株粘质沙雷氏菌分离株，这些菌株于1980年至1995年期间从50名患者体内分离得到，在细菌素（噬菌体尾）敏感性、碳源同化或血清型方面表现出表型变异，现用这三种表型方法对其进行重新检测。7株分离株（5.4%）无法进行细菌素分型；所有129株分离株均能利用碳源且可进行血清分型。来自20名患者的48株分离株用这三种表型方法得出了明确结果，被排除在进一步分析之外。在其余来自30名患者的81株分离株中，来自2名患者的分离株仅在细菌素敏感性方面表现出表型变异，而来自6名患者的分离株显示出不同的细菌素类型和生化谱，但血清型相同。来自20名患者的分离株仅显示出生化谱变异。3名患者发生了不同表型和基因型的粘质沙雷氏菌菌株的重叠感染。在4名患者中，先前有运动性（H12）的分离株变为无运动性（H-）。对30名患者的81株分离株经XbaI和SpeI酶切的基因组DNA进行PFGE分析，结果显示22名患者的分离株基因型相同。来自3名患者的分离株基因型密切相关，另一名患者的分离株可能相关。PFGE分析显示一名患者感染了两种血清型相同但细菌素类型和生化谱不同的基因型不同的粘质沙雷氏菌菌株。得出的结论是，对粘质沙雷氏菌限制性基因组DNA进行PFGE分析优于所比较的三种表型方法。血清分型比细菌素分型更可靠，并且在从长期感染粘质沙雷氏菌的患者连续分离得到的菌株中，细菌素分型技术产生的表型变异比生化谱测定少。
