von Graevenitz A, Funke G
Department of Medical Microbiology, University of Zurich, Switzerland.
Zentralbl Bakteriol. 1996 Jul;284(2-3):246-54. doi: 10.1016/s0934-8840(96)80100-9.
An identification scheme for aerobically growing Gram-positive rods (genera Actinomyces, Arcanobacterium, Aureobacterium, Bacillus, Brevibacterium, Cellulomonas, Corynebacterium, Dermabacter, Erysipelothrix, Gardnerella, Lactobacillus, Listeria, Microbacterium, Oerskovia, Propionibacterium, Rhodococcus, Rothia, Turicella, as well as unnamed CDC groups, Clostridium tertium, and Mycobacterium fortuitum/chelonae) is presented. It is derived from the Hollis-Weaver scheme and uses catalase, oxidative/fermentative carbohydrate metabolism and motility as primary reactions. Tests for lipophilism, nitrate reduction, urease, esculin hydrolysis, the CAMP reaction, acid formation from five carbohydrates, as well as for some facultative reactions should lead to a correct diagnosis based on information available at the end of 1995.
本文介绍了一种用于需氧生长的革兰氏阳性杆菌(放线菌属、隐秘杆菌属、金色杆菌属、芽孢杆菌属、短杆菌属、纤维单胞菌属、棒状杆菌属、皮肤杆菌属、丹毒丝菌属、加德纳菌属、乳杆菌属、李斯特菌属、微杆菌属、厄氏菌属、丙酸杆菌属、红球菌属、罗氏菌属、图里塞尔菌属,以及未命名的疾控中心菌群、第三梭菌和偶然分枝杆菌/龟分枝杆菌)的鉴定方案。该方案源自霍利斯-韦弗方案,以过氧化氢酶、氧化/发酵碳水化合物代谢和运动性作为主要反应。基于1995年底所掌握的信息,通过对嗜脂性、硝酸盐还原、尿素酶、七叶苷水解、CAMP反应、五种碳水化合物产酸情况以及一些兼性反应的检测,应能得出正确诊断。