Haber-Mignard D, Suschetet M, Bergès R, Astorg P, Siess M H
Unité de Toxicologie Nutritionnelle, Institut National de la Recherche Agronomique, Dijon, France.
Nutr Cancer. 1996;25(1):61-70. doi: 10.1080/01635589609514428.
The anti-initiating properties of allyl sulfides on rat liver carcinogenesis induced by N-nitrosodiethylamine (NDEA) or aflatoxin B1 (AFB1) were evaluated by using a three-step medium-term hepatocarcinogenesis assay. Diallyl sulfide (DAS) or diallyl disulfide (DADS) was added to the diet of rats (2 g/kg) for three weeks, during which NDEA or AFB1 was administered by intraperitoneal injection. The rats were submitted later to eight days of 2-acetylaminofluorene administration and to two-thirds hepatectomy, then to phenobarbital administration. After eight weeks, liver preneoplastic foci expressing the placental form of glutathione S-transferase were detected. The results show that DAS and DADS strongly reduced the number and the size of preneoplastic foci initiated by NDEA and AFB1, but especially by AFB1; DADS is more efficient than DAS. Most likely, the inhibition of the first step of hepatocarcinogenesis by allyl sulfides is related to the modulating effects that these compounds exert on the enzymes involved in activation and/or detoxication of the carcinogens. Our study demonstrated the chemopreventive potencies of dietary allyl sulfides in liver carcinogenesis induced by two potent hepatic carcinogens.
采用三步中期肝癌发生试验，评估了烯丙基硫化物对N-亚硝基二乙胺（NDEA）或黄曲霉毒素B1（AFB1）诱导的大鼠肝癌发生的抗启动特性。将二烯丙基硫化物（DAS）或二烯丙基二硫化物（DADS）以2 g/kg的剂量添加到大鼠饮食中，持续三周，在此期间通过腹腔注射给予NDEA或AFB1。随后，对大鼠进行为期八天的2-乙酰氨基芴给药和三分之二肝切除术，然后给予苯巴比妥。八周后，检测到表达谷胱甘肽S-转移酶胎盘形式的肝脏癌前病灶。结果表明，DAS和DADS能显著减少由NDEA和AFB1引发的癌前病灶的数量和大小，尤其是AFB1引发的；DADS比DAS更有效。烯丙基硫化物对肝癌发生第一步的抑制作用很可能与这些化合物对参与致癌物激活和/或解毒的酶的调节作用有关。我们的研究证明了膳食烯丙基硫化物在两种强效肝脏致癌物诱导的肝癌发生中的化学预防潜力。
