Vliegen H W, Eulderink F, Bruschke A V, van der Laarse A, Cornelisse C J
Department of Cardiology, University of Leiden, Netherlands.
Am J Cardiovasc Pathol. 1995;5(1):27-31.
Increased heart weight is known to be associated with polyploidy of myocardial nuclei. However, the relation between polyploidy and myocardial hypertrophy at a cellular level, i.e. myocyte hypertrophy, has not yet been investigated. The present study was conducted to reveal this relation in different regions in the heart using two recently developed techniques, i.e. flow cytometry to measure polyploidy and microscopic morphometry to measure myocyte dimensions in fixed tissue sections. We investigated the effect of pressure overload induced hypertrophy in subendocardial and subepicardial regions of 43 human left ventricles and transmural samples from 47 human right ventricles. In all regions the degree of polyploidy correlated well with myocyte volume indexed for body surface area. At equal extent of myocyte hypertrophy, the degree of polyploidy was higher in left ventricular samples than in right ventricular samples. In conclusion, pressure overload induced myocyte hypertrophy is associated with polyploidy, which is more pronounced in the left ventricle than in the right ventricle.
已知心脏重量增加与心肌细胞核多倍体有关。然而，多倍体与细胞水平的心肌肥大，即心肌细胞肥大之间的关系尚未得到研究。本研究采用两种最新开发的技术，即流式细胞术测量多倍体和显微镜形态测量法测量固定组织切片中的心肌细胞尺寸，以揭示心脏不同区域的这种关系。我们研究了压力超负荷诱导的肥大对43个人类左心室的心内膜下和心外膜下区域以及47个人类右心室的透壁样本的影响。在所有区域，多倍体程度与以体表面积指数化的心肌细胞体积密切相关。在心肌细胞肥大程度相同的情况下，左心室样本中的多倍体程度高于右心室样本。总之，压力超负荷诱导的心肌细胞肥大与多倍体有关，左心室比右心室更明显。
