Singh R K, Llansa N, Bucana C D, Sanchez R, Koura A, Fidler I J
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Cell Growth Differ. 1996 Mar;7(3):397-404.
We investigated some of the mechanisms that regulate the expression of basic fibroblast growth factor (bFGF) in human renal cell carcinoma (HRCC). HRCC SN12PM6 cells were cultured as adherent monolayers. The expression of steady-state bFGF mRNA (measured by in situ hybridization and Northern blot) and protein (measured by immunohistochemistry and ELISA) correlated inversely with the culture density. Tumor cells harvested from dense cultures (low bFGF expression) and plated under sparse conditions expressed high levels of bFGF mRNA and protein prior to cell division, suggesting that bFGF may be a competence factor. Similar data were obtained with human vascular endothelial cells. The expression of bFGF was not regulated by spent culture medium, cell cycle, or rate of cell division but was down-regulated by contract inhibition. These data show that the expression of bFGF in HRCC is cell density dependent.
我们研究了一些调节人肾细胞癌（HRCC）中碱性成纤维细胞生长因子（bFGF）表达的机制。HRCC SN12PM6细胞以贴壁单层培养。稳态bFGF mRNA（通过原位杂交和Northern印迹法测定）和蛋白质（通过免疫组织化学和ELISA测定）的表达与培养密度呈负相关。从高密度培养物（低bFGF表达）中收获并接种在稀疏条件下的肿瘤细胞在细胞分裂前表达高水平的bFGF mRNA和蛋白质，这表明bFGF可能是一种感受态因子。用人血管内皮细胞也获得了类似的数据。bFGF的表达不受用过的培养基、细胞周期或细胞分裂速率的调节，但受接触抑制的下调。这些数据表明，HRCC中bFGF的表达是细胞密度依赖性的。
