Zhong H, Deng F, Kong X
Institute of Urology, Beijing Medical University.
Zhonghua Wai Ke Za Zhi. 1996 Nov;34(11):651-4.
Basic fibroblast growth factor (bFGF) is a pluripotent polypeptide which plays an important role in tumor progression and angiogensis. We determined the expression and localization patterns of bFGF and one of its receptor (FGFR-1) in normal renal as well as in renal cancers. The results were compared with clinicopathologic features. Using bFGF and FGFR-1 antibody, the repairing method of antigen with microwave oven heating and LSAB immunohistochemistry we used in 36 cases of paraffin-embedded renal cell carcinoma (RCC) and their paired normal renal tissues. The expression of bFGF and FGFR-1 was nearly consistent. The normal renal tissues and ECM of 29 cases of renal cancer tissues showed heterogenous immunoreactivities. Renal cancer cell cytoplasm of 12 primary tumors and 2 metastatic tumors, as in the cytoplasmic bFGF of cultured GRC-1 cells, were positively homogenous stained. The bFGF and FGFR-1 can be consistently expressed in normal renal and renal cancer tissues, reflecting that the expression and function of these substances were closely associated. The cytoplasmic bFGF expression of renal cancer was related to tumor stages, suggesting that b bFGF plays an important role in the progression of renal cell carcinoma.
碱性成纤维细胞生长因子（bFGF）是一种多能性多肽，在肿瘤进展和血管生成中发挥重要作用。我们测定了bFGF及其一种受体（FGFR-1）在正常肾脏组织以及肾癌组织中的表达和定位模式。将结果与临床病理特征进行比较。我们采用bFGF和FGFR-1抗体，运用微波炉加热抗原修复法和LSAB免疫组织化学方法，对36例石蜡包埋的肾细胞癌（RCC）及其配对的正常肾组织进行检测。bFGF和FGFR-1的表达情况基本一致。29例肾癌组织的正常肾组织和细胞外基质呈现出异质性免疫反应。12例原发性肿瘤和2例转移瘤的肾癌细胞胞质，如同培养的GRC-1细胞胞质中的bFGF一样，呈阳性均匀染色。bFGF和FGFR-1在正常肾组织和肾癌组织中均可持续表达，这反映出这些物质的表达与功能密切相关。肾癌的胞质bFGF表达与肿瘤分期相关，提示bFGF在肾细胞癌的进展中发挥重要作用。
