Castilho R F, Pereira R S, Vercesi A E
Departamento de Bioquímica, IB, UNICAMP, Campinas, São Paulo, Brazil.
Eur J Drug Metab Pharmacokinet. 1996 Jan-Mar;21(1):17-21. doi: 10.1007/BF03190273.
In this study, we show that safranine at the concentrations usually employed as a probe of mitochondrial membrane potential significantly protects against the oxidative damage of mitochondria induced by Fe(II)citrate. The effect of safranine was illustrated by experiments showing that this dye strongly inhibits both production of thiobarbituric acid-reactive substances and membrane potential decrease when energized mitochondria were exposed to Fe(II)citrate in the presence of Ca2+ ions. Similar results were obtained with the lipophylic compound trifluoperazine. It is proposed that, like trifluoperazine, safranine decreases the rate of lipid peroxidation due to its insertion in the membrane altering the physical state of the lipid phase.
在本研究中，我们发现，通常用作线粒体膜电位探针的番红精在其常用浓度下能显著保护线粒体免受柠檬酸亚铁诱导的氧化损伤。番红精的作用通过实验得以阐明，这些实验表明，当在Ca2+离子存在的情况下将供能线粒体暴露于柠檬酸亚铁时，这种染料能强烈抑制硫代巴比妥酸反应性物质的生成以及膜电位的降低。亲脂性化合物三氟拉嗪也得到了类似结果。有人提出，与三氟拉嗪一样，番红精由于插入膜中改变了脂质相的物理状态，从而降低了脂质过氧化速率。
