Cortadas J, Subirana J A
Biochim Biophys Acta. 1977 Jun 3;476(3):203-6. doi: 10.1016/0005-2787(77)90003-x.
In this paper we show that the extent of DNA denaturation achieved by dialysis against N,N-dimethyl formamide depends on both the source of DNA and the buffer used. The stability against denaturation increases with the guanine plus cytosine content. The base sequence also plays a minor role. This behavior is due to differences in the solubility of DNA in the denaturing solvent. The DNAs with a high guanine-cytosine content have a lower solubility in dimethyl formamide and this fact results in a higher stability against denaturation. As a practical consequence, in order to achieve complete denaturation of DNA by dialysis against dimethyl formamide, it is necessary to start with DNA dissolved in a buffer of low ionic strength, preferably below 10(-3) M.
在本文中，我们表明通过对N，N - 二甲基甲酰胺进行透析所达到的DNA变性程度取决于DNA的来源和所使用的缓冲液。抗变性的稳定性随鸟嘌呤加胞嘧啶含量的增加而提高。碱基序列也起次要作用。这种行为是由于DNA在变性溶剂中的溶解度不同所致。鸟嘌呤 - 胞嘧啶含量高的DNA在二甲基甲酰胺中的溶解度较低，这一事实导致其具有更高的抗变性稳定性。实际结果是，为了通过对二甲基甲酰胺进行透析实现DNA的完全变性，有必要从溶解于低离子强度缓冲液（最好低于10^(-3) M）中的DNA开始。
