哺乳动物DNA的高分辨率热变性
High resolution thermal denaturation of mammalian DNAs.
作者信息
Guttmann T, Vítek A, Pivec L
出版信息
Nucleic Acids Res. 1977 Feb;4(2):285-97. doi: 10.1093/nar/4.2.285.
Abstract
High resolution melting profiles of different mammalian DNAs are presented. Melting curves of various mammalian DNAs were compared with respect to the degree of asymmetry, first moment, transition breath and Tmi of individual subtransitions. Quantitative comparison of the shape of all melting curves was made. Correlation between phylogenetical relations among mammals and shape of the melting profiles of their DNAs was demonstrated. The difference between multi-component heterogeneity of mammalian DNAs found by optical melting analysis and sedimentation in CsCl-netropsin density gradient is also discussed.
摘要
本文展示了不同哺乳动物DNA的高分辨率熔解图谱。比较了各种哺乳动物DNA熔解曲线在不对称程度、一阶矩、转变宽度和各个亚转变的熔解温度方面的差异。对所有熔解曲线的形状进行了定量比较。证明了哺乳动物之间的系统发育关系与其DNA熔解图谱形状之间的相关性。还讨论了通过光学熔解分析和氯化铯-纺锤菌素密度梯度沉降发现的哺乳动物DNA多组分异质性之间的差异。
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