Tarutani O, Kondo T, Shulman S
Biochim Biophys Acta. 1977 Jun 24;492(2):284-99. doi: 10.1016/0005-2795(77)90079-4.
In studying the solubility characteristics of thyroglobulin, it was found that the removal of sialic acid residues from either human or hog thyroglobulin by treatment with neuraminidase markedly decreased the solubility in salt solutions. On the other hand, naturally sialic acid-rich thyroglobulin obtained from human diffuse goiter was slightly more soluble than thyroglobulin in which sialic acid content was within the normal range. However, the desialized preparations prepared from both normal and sialic acid-rich thyroglobulin showed markedly lower solubility and further, showed no significant difference in solubility between them. These results indicate that the surface charge of thyroglobulin which influenced the salting-out property depends largely on the number of sialic acid residues in a thyroglobulin molecule, not on the degree of iodination of thyroglobulin.
在研究甲状腺球蛋白的溶解性特征时发现，用神经氨酸酶处理人或猪的甲状腺球蛋白以去除唾液酸残基，会显著降低其在盐溶液中的溶解度。另一方面，从人弥漫性甲状腺肿中获得的天然富含唾液酸的甲状腺球蛋白比唾液酸含量在正常范围内的甲状腺球蛋白的溶解度略高。然而，由正常甲状腺球蛋白和富含唾液酸的甲状腺球蛋白制备的去唾液酸制剂的溶解度明显更低，而且，它们之间的溶解度没有显著差异。这些结果表明，影响盐析性质的甲状腺球蛋白表面电荷在很大程度上取决于甲状腺球蛋白分子中唾液酸残基的数量，而不是甲状腺球蛋白的碘化程度。
