蓖麻低分子量种子蛋白的分离与鉴定
Isolation and characterization of a low-molecular weight seed protein from Ricinus communis.
作者信息
Li S S, Lin T T, Forde M D
出版信息
Biochim Biophys Acta. 1977 Jun 24;492(2):364-9. doi: 10.1016/0005-2795(77)90087-3.
PMID:884136
Abstract
A seed protein with an apparent molecular weight of 5000 has been isolated and purified from Ricinus communis. This protein has a very high content of glutamine and an unusual ultraviolet absorption spectrum. The amino-terminal sequence of 22 residues determined by automatic Edman degradation is: NH2-Pro-Ser-Gln-Gln-Gly-Cys-Cys-Gly-Gln-Ile-Gln-Glu-Gln-Gln-Asn-Leu-Arg-Gln-Cys-Gln-Glu-Tyr-.
摘要
从蓖麻中分离并纯化出一种表观分子量为5000的种子蛋白。这种蛋白质含有非常高的谷氨酰胺含量以及不寻常的紫外吸收光谱。通过自动埃德曼降解法测定的22个残基的氨基末端序列为：NH2-脯氨酸-丝氨酸-谷氨酰胺-谷氨酰胺-甘氨酸-半胱氨酸-半胱氨酸-甘氨酸-谷氨酰胺-异亮氨酸-谷氨酰胺-谷氨酸-谷氨酰胺-谷氨酰胺-天冬酰胺-亮氨酸-精氨酸-谷氨酰胺-半胱氨酸-谷氨酰胺-谷氨酸-酪氨酸- 。
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