上游DNA弯曲对单纯疱疹病毒胸苷激酶启动子强度的影响。
Effects of an upstream DNA curvature on the strength of HSV thymidine kinase promoter.
作者信息
Ohyama T, Kadokawa Y, Hirota Y
机构信息
Department of Biology, Faculty of Science, Konan University, Kobe, Japan.
出版信息
Nucleic Acids Symp Ser. 1995(34):67-8.
PMID:8841555
Abstract
The effect of an upstream DNA curvature on the strength of the herpes simplex virus thymidine kinase (TK) promoter was investigated by employing luciferase assay. A set of double-stranded oligonucleotides, each with an unique DNA configuration, were synthesized and substituted into an upstream region of the promoter. It was revealed from the assay that both plane and space curves exerted a negative influence upon the strength of the promoter.
摘要
通过荧光素酶测定法研究了上游DNA曲率对单纯疱疹病毒胸苷激酶（TK）启动子强度的影响。合成了一组双链寡核苷酸，每个都具有独特的DNA构型，并将其替换到启动子的上游区域。该测定结果表明，平面曲线和空间曲线均对启动子强度产生负面影响。
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