Zama M
National Institute of Radiobiological Sciences, Chiba-shi, Japan.
Nucleic Acids Symp Ser. 1995(34):97-8.
It has recently been reported that ribosomes pause at specific sites during translation of the chloroplast photosystem II reaction center protein D1(1). We have obtained the stability map of local secondary structure of the mRNA of psbA gene, encoding for D1, by plotting the free energy of the optimal secondary structure of the local segment of the mRNA against the segment position along a base sequence of the mRNA. It was found that the positions of minima or lower free energy in the map coincide with the positions of the pausing sites during synthesis of D1. The result suggests that synthesis pauses of D1 could-be induced by the mRNA secondary structure.
最近有报道称，核糖体在叶绿体光系统II反应中心蛋白D1的翻译过程中会在特定位点暂停(1)。我们通过绘制mRNA局部片段的最佳二级结构的自由能与mRNA碱基序列上片段位置的关系图，获得了编码D1的psbA基因mRNA的局部二级结构稳定性图谱。结果发现，图谱中自由能最小值或较低自由能的位置与D1合成过程中的暂停位点位置一致。这一结果表明，D1合成的暂停可能是由mRNA二级结构诱导的。
