用于核磁共振信号归属的RNA选择性氘代
Selective deuteration of RNA for NMR signal assignment.
作者信息
Kim I, Watanabe S, Muto Y, Hosono K, Takai K, Takaku H, Kawai G, Watanabe K, Yokoyama S
机构信息
Department of Biophysics and Biochemistry, School of Science, University of Tokyo, Japan.
出版信息
Nucleic Acids Symp Ser. 1995(34):123-4.
For site-selective deuterium labeling of RNA, [5-2H]uridine phosphoramidite was prepared. The uridine at position 10 of a 25-mer RNA, GGACAGACUUCGGUCGGAGUACUCG, was labeled in two different manners for "positive" (U = [5-1H]U, U = [5-2H] U) and "negative"(U = [5-2H]U, U = [5-1H]U) observations. By comparison of NOESY spectra of the two labeled samples with that of the unlabeled RNA, we could unambiguously assign the H5-H6 signals of U10, and measure their NOE connectivities.
为了实现RNA的位点选择性氘代标记，制备了[5-2H]尿苷亚磷酰胺。对25聚体RNA（GGACAGACUUCGGUCGGAGUACUCG）第10位的尿苷进行了两种不同方式的标记，以进行“正”（U = [5-1H]U，U = [5-2H]U）和“负”（U = [5-2H]U，U = [5-1H]U）观测。通过比较两个标记样品的NOESY谱与未标记RNA的NOESY谱，我们能够明确归属U10的H5-H6信号，并测量它们的NOE连接性。
Zotero 插件