Hutter-Paier B, Grygar E, Windisch M
Center of Animal Biology, Medical School, University of Graz, Austria.
J Neural Transm Suppl. 1996;47:267-73. doi: 10.1007/978-3-7091-6892-9_19.
Glutamate induced neurotoxicity has been proposed to account for the loss of neurons after ischemia as well as in the cause of neurodegenerative diseases. We have studied the effects of exogenous glutamate on survival of neurons from chick embryo telencephalon, precultured with a peptide derivative for 8 days. The peptide derivative Cerebrolysin is a drug produced by standardised enzymatic breakdown consisting of 80% peptides and 20% amino acids. Toxic effects of acute glutamate exposure were prevented by Cerebrolysin in a concentration-dependent manner. 20 and 40 microliters Cerebrolysin produce distinct neuroprotective effects. However, 80 microliters Cerebrolysin/ml nutrition medium more than doubles neuronal viability compared to untreated control cells. These concentration-dependent effects of Cerebrolysin were evident even at the light microscopic level.
谷氨酸诱导的神经毒性被认为是缺血后神经元丧失以及神经退行性疾病病因的原因。我们研究了外源性谷氨酸对鸡胚端脑神经元存活的影响，这些神经元预先用一种肽衍生物培养了8天。肽衍生物脑活素是一种通过标准化酶解产生的药物，由80%的肽和20%的氨基酸组成。脑活素以浓度依赖的方式预防了急性谷氨酸暴露的毒性作用。20微升和40微升脑活素产生明显的神经保护作用。然而，与未处理的对照细胞相比，每毫升营养培养基中80微升脑活素使神经元活力增加了一倍多。脑活素的这些浓度依赖性作用即使在光学显微镜水平也很明显。
