Taiji M, Tohdoh N, Ojika K
Discovery Research Laboratories II, Sumitomo Pharmaceuticals Research Center, Osaka, Japan.
J Neurosci Res. 1996 Aug 1;45(3):202-15. doi: 10.1002/(SICI)1097-4547(19960801)45:3<202::AID-JNR2>3.0.CO;2-H.
The expression of hippocampal cholinergic neurostimulating peptide (HCNP)-precursor mRNA in rat brain was examined by Northern blot and in situ hybridization analyses. Northern blot analysis using rat HCNP-precursor cDNA revealed a 1.1-kilobase (kb) transcript. A message of identical size was also detected with the antisense precursor riboprobe. In situ hybridization disclosed that HCNP-precursor mRNA was expressed in many areas of the brain, including the basal forebrain cholinergic system, the olfactory system, and the cerebellum. Very high levels were seen in the pyramidal cells of the CA3 region and in the hilus of the dentate gyrus of the hippocampal formation. High levels were also found in the septal area, piriform cortex, entorhinal cortex, thalamic nuclei, subthalamic nuclei, medial habenular nuclei, substantia nigra, Purkinje cells of the cerebellum, and choroid plexus. By contrast, glial cells were not labeled by the antisense HCNP-precursor riboprobe. The expression of HCNP-precursor mRNA by a variety of neurons suggests that HCNP and its precursor protein play significant roles in the stimulation of cholinergic activity, as well as in other not yet defined functions.
通过Northern印迹和原位杂交分析检测了大鼠脑海马胆碱能神经刺激肽（HCNP）前体mRNA的表达。使用大鼠HCNP前体cDNA进行的Northern印迹分析显示出一个1.1千碱基（kb）的转录本。用反义前体核糖探针也检测到了相同大小的信息。原位杂交显示，HCNP前体mRNA在大脑的许多区域表达，包括基底前脑胆碱能系统、嗅觉系统和小脑。在海马结构CA3区的锥体细胞和齿状回的门区中可见非常高水平的表达。在隔区、梨状皮质、内嗅皮质、丘脑核、丘脑底核、内侧缰核、黑质、小脑的浦肯野细胞和脉络丛中也发现了高水平的表达。相比之下，反义HCNP前体核糖探针未标记胶质细胞。多种神经元表达HCNP前体mRNA表明，HCNP及其前体蛋白在刺激胆碱能活性以及其他尚未明确的功能中发挥着重要作用。
