Ojika K, Katada E, Tohdoh N, Mitake S, Otsuka Y, Matsukawa N, Tsugu Y
Second Department of Internal Medicine, Medical School, Nagoya City University, Japan.
Brain Res. 1995 Dec 1;701(1-2):19-27. doi: 10.1016/0006-8993(95)00951-6.
This report concerns the demonstration of hippocampal cholinergic neurostimulating peptide (HCNP), its deacetylated analogue (free HCNP) and HCNP precursor protein in rat tissues. To avoid possible enzymatic degradation during sample manipulation, tissue extracts were prepared under acidic conditions using trifluoroacetic acid. The tissue contents of free HCNP and of precursor protein were determined by radioimmuno-assay (RIA) using two antibodies with different specificities, and by a combination of HPLC and RIA. Free HCNP was detected in neuronal and renal tissues, but not in liver. All tissues examined had measurable amounts of HCNP precursor protein. The concentrations of free HCNP and precursor in neuronal tissues were inversely related to the age. These results suggest that the deacetylated analogue of HCNP and its precursor protein may have significant physiological functions, especially in the central nervous system of young animals.
本报告涉及大鼠组织中海马胆碱能神经刺激肽（HCNP）、其去乙酰化类似物（游离HCNP）和HCNP前体蛋白的检测。为避免样品处理过程中可能的酶降解，使用三氟乙酸在酸性条件下制备组织提取物。游离HCNP和前体蛋白的组织含量通过使用两种具有不同特异性的抗体的放射免疫分析（RIA）以及HPLC和RIA的组合来测定。在神经组织和肾组织中检测到游离HCNP，但在肝脏中未检测到。所有检测的组织都有可测量量的HCNP前体蛋白。神经组织中游离HCNP和前体的浓度与年龄呈负相关。这些结果表明，HCNP的去乙酰化类似物及其前体蛋白可能具有重要的生理功能，尤其是在幼龄动物的中枢神经系统中。
