Kurek J, Bower J, Romanella M, Austin L
Melbourne Neuromuscular Research Centre, St. Vincent's Hospital, Fitzroy, Victoria, Australia.
Neurosci Lett. 1996 Jul 19;212(3):167-70. doi: 10.1016/0304-3940(96)12802-0.
A number of growth factors are involved in coordinating muscle cell proliferation and differentiation, particularly after injury and in disease. Leukaemia inhibitory factor (LIF) strongly stimulates the proliferation of myoblasts in vitro and in vivo and its expression in muscle after injury suggests that LIF may have a role as a trauma factor. The mdx mouse was used to study the effects of LIF on in vivo muscle regeneration during disease. The rationale for using trophic factors such as LIF to treat neuromuscular disease includes the understanding that these molecules show some degree of selectivity for the population of cells in which they are effective. LIF was administered to muscle of the mdx mouse using osmotic pumps implanted subcutaneously in unrestrained mice. The growth factor was continuously delivered into the vastus lateralis muscle at 7 U/mu 1 for 7 days via a catheter. The results show that LIF increased the rate of muscle regeneration in mdx mice by stimulating the formation of larger myotubes. LIF treatment also increased the number of regenerating myotubes in the perfused area. This myotrophic action indicates that LIF contributes to muscle regeneration. Together with its known neurotrophic action, LIF is a potential therapeutic agent for the treatment of neuromuscular disease.
许多生长因子参与协调肌肉细胞的增殖和分化，尤其是在受伤后和疾病状态下。白血病抑制因子（LIF）在体外和体内均能强烈刺激成肌细胞的增殖，且其在受伤后肌肉中的表达表明LIF可能作为一种创伤因子发挥作用。mdx小鼠被用于研究LIF在疾病过程中对体内肌肉再生的影响。使用诸如LIF等营养因子治疗神经肌肉疾病的基本原理包括，人们认识到这些分子对其有效作用的细胞群体具有一定程度的选择性。通过将渗透泵皮下植入无束缚的小鼠体内，将LIF注入mdx小鼠的肌肉。生长因子通过导管以7 U/μl的浓度持续7天输送到股外侧肌中。结果表明，LIF通过刺激更大肌管的形成，提高了mdx小鼠的肌肉再生速率。LIF治疗还增加了灌注区域中再生肌管的数量。这种促肌营养作用表明LIF有助于肌肉再生。连同其已知的神经营养作用，LIF是治疗神经肌肉疾病的一种潜在治疗剂。
