Lopshire R F, Watson J T, Enke C G
Department of Chemistry, Michigan State University, East Lansing, USA.
Toxicol Ind Health. 1996 May-Aug;12(3-4):375-91. doi: 10.1177/074823379601200310.
The exchange reaction of chlorine by oxygen with polychlorinated biphenyl (PCB) anions was explored as a method for composition-selective detection of PCB congeners in a gas chromatograph/tandem mass spectrometer (GC/MS/MS) experiment. The odd-electron molecular anions of PCBs react with molecular oxygen introduced into the collision cell of a triple-quadrupole mass spectrometer to form a product ion that has 19 mass units less (35Cl - 16O) than the precursor. The even-electron anions which also form in the source do not react when allowed to pass through the precursor analyzer. Multiple reaction monitoring allows the generation of separate chromatograms for each different composition from tetrachloro through nonochloro PCB. Most coelutants are resolved in this way. Response factors for the 12 coplanar congeners that exhibit dioxin-like toxicity relative to octachloronaphthalene vary from 1.6 to 580. Results of the analysis of an Aroclor 1242 sample for the coplanar congeners compare well with previously determined values.
在气相色谱/串联质谱（GC/MS/MS）实验中，研究了用氧与多氯联苯（PCB）阴离子进行氯交换反应，以此作为一种对PCB同系物进行成分选择性检测的方法。多氯联苯的奇电子分子阴离子与引入到三重四极杆质谱仪碰撞池中的分子氧发生反应，形成一种产物离子，该产物离子的质量比前体离子少19个质量单位（35Cl - 16O）。源中同样形成的偶电子阴离子在通过前体分析仪时不发生反应。多反应监测允许为从四氯到九氯PCB的每种不同成分生成单独的色谱图。大多数共流出物以这种方式得以分离。相对于八氯萘，12种具有二噁英样毒性的共面同系物的响应因子在1.6到580之间变化。对Aroclor 1242样品中共面同系物的分析结果与先前测定的值比较吻合。
