三种基因的过量表达导致大肠杆菌对樟脑产生抗性并出现染色体凝聚。
Overproduction of three genes leads to camphor resistance and chromosome condensation in Escherichia coli.
作者信息
Hu K H, Liu E, Dean K, Gingras M, DeGraff W, Trun N J
机构信息
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
出版信息
Genetics. 1996 Aug;143(4):1521-32. doi: 10.1093/genetics/143.4.1521.
Abstract
We isolated and characterized three genes, crcA, cspE and crcB, which when present in high copy confer camphor resistance on a cell and suppress mutations in the chromosomal partition gene mukB. Both phenotypes require the same genes. Unlike chromosomal camphor resistant mutants, high copy number crcA, cspE and crcB do not result in an increase in the ploidy of the cells. The cspE gene has been previously identified as a cold shock-like protein with homologues in all organisms tested. We also demonstrate that camphor causes the nucleoids to decondense in vivo and when the three genes are present in high copy, the chromosomes do not decondense. Our results implicate camphor and mukB mutations as interfering with chromosome condensation and high copy crcA, cspE and crcB as promoting or protecting chromosome folding.
摘要
我们分离并鉴定了三个基因，即crcA、cspE和crcB，当它们以高拷贝形式存在时，能赋予细胞对樟脑的抗性，并抑制染色体分配基因mukB中的突变。这两种表型需要相同的基因。与染色体樟脑抗性突变体不同，高拷贝数的crcA、cspE和crcB不会导致细胞倍性增加。cspE基因先前已被鉴定为一种冷休克样蛋白，在所测试的所有生物体中都有同源物。我们还证明，樟脑会导致类核在体内解聚，而当这三个基因以高拷贝形式存在时，染色体不会解聚。我们的结果表明，樟脑和mukB突变会干扰染色体凝聚，而高拷贝的crcA、cspE和crcB则促进或保护染色体折叠。
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