Clutter A C, Pomp D, Murray J D
Department of Animal Science, Oklahoma State University, Stillwater 74078-0425, USA.
Genetics. 1996 Aug;143(4):1753-60. doi: 10.1093/genetics/143.4.1753.
Transgenic mice possessing an ovine growth hormone gene were used to study the effects of elevated growth hormone on quantitative genetic variation. Males hemizygous for the transgene were mated to wild-type females to produce half- and full-sib families in which approximately half the progeny were transgenic and half were wild type. Analyses of body weights at 3-10 weeks, and weight gains from 3 to 6, and 6 to 10 weeks produced estimates of the proportion of total variance due to additive genetic effects (h2) and common litter effects (c2), and the genetic correlation between transgenic and wild-type expression of each trait. At 10 weeks, body weight of transgenics exceeded that of wild types by 26 and 49% in males and females, respectively. Estimated genetic variances in the transgenic group were significantly greater than zero for body weights at most ages and for both measurements of gain. Common litter effects accounted for a similar proportion of variation in the wild-type and transgenic groups. Additive genetic correlations between wild-type and transgenic expression of body weights tended to decline with age, indicating that a partially different array of genes may have begun to affect body weight in the transgenic group.
利用携带绵羊生长激素基因的转基因小鼠来研究生长激素水平升高对数量遗传变异的影响。将转基因半合子雄性小鼠与野生型雌性小鼠交配，以产生半同胞和全同胞家系，其中约一半后代为转基因小鼠，另一半为野生型小鼠。对3至10周龄的体重以及3至6周和6至10周的体重增加情况进行分析，得出了由于加性遗传效应（h2）和共同窝效应（c2）导致的总方差比例估计值，以及每个性状转基因表达与野生型表达之间的遗传相关性。在10周龄时，转基因雄性和雌性小鼠的体重分别比野生型小鼠超出26%和49%。在大多数年龄段，转基因组的体重估计遗传方差显著大于零，且在两种增重测量中也是如此。共同窝效应在野生型和转基因组中占变异的比例相似。体重的野生型表达与转基因表达之间的加性遗传相关性倾向于随年龄下降，这表明在转基因组中，可能开始有一部分不同的基因阵列影响体重。
