Latimer K S, Niagro F D, Steffens W L, Ritchie B W, Campagnoli R P
Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602-7388, USA.
J Vet Diagn Invest. 1996 Jul;8(3):291-5. doi: 10.1177/104063879600800303.
Necropsy tissues were examined from an adult wild-caught Ducorps' cockatoo (Cacatua ducorpsii) with progressive neurologic signs. Of the tissue specimens selected for histologic evaluation, only the brain contained rare amphophilic, glassy intranuclear inclusions within astrocytes and some neurons. Astrocyte and neuronal degeneration and necrosis also were observed. Scattered astrocytes, with and without discernable inclusions, contained avian polyomavirus (APV) nucleic acid, as determined by DNA in situ hybridization. In addition, endothelial cells and intravascular leukocytes contained psittacine beak and feather disease viral nucleic acid, as determined by DNA in situ hybridization, indicating dual viral infection. Electron microscopic examination of formalin-fixed brain tissue revealed typical intranuclear APV particles in some astrocytes. Encephalopathy ultimately was attributed to APV infection.
对一只成年野生捕获的杜氏凤头鹦鹉（Cacatua ducorpsii）进行尸检，该鹦鹉有进行性神经症状。在选取用于组织学评估的组织标本中，只有大脑在星形胶质细胞和一些神经元内含有罕见的嗜双色性、玻璃样核内包涵体。还观察到星形胶质细胞和神经元变性及坏死。通过DNA原位杂交确定，散在的星形胶质细胞，无论有无可识别的包涵体，均含有禽多瘤病毒（APV）核酸。此外，通过DNA原位杂交确定，内皮细胞和血管内白细胞含有鹦鹉喙羽病病毒核酸，表明存在双重病毒感染。对福尔马林固定的脑组织进行电子显微镜检查，在一些星形胶质细胞中发现了典型的核内APV颗粒。脑病最终归因于APV感染。
