Garcia A, Latimer K S, Niagro F D, Ritchie B W, Campagnoli R P
Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602.
J Vet Diagn Invest. 1994 Jul;6(3):308-14. doi: 10.1177/104063879400600305.
Liver sections from 32 psittacine birds with multifocal to coalescing hepatocellular necrosis were examined to determine the cause of disease. Avian polyomavirus (APV) infection (19 of 32 birds), bacterial hepatitis (5 of 32 birds), and chlamydiosis (3 of 32 birds) were major causes of hepatic disease. The presence of APV inclusions or nucleic acid was demonstrated using hematoxylin and eosin (HE) staining, DNA in situ hybridization, and DNA amplification with Southern blotting. Amphophilic intranuclear inclusions, suggestive of APV infection, were observed in HE-stained liver sections from 5 of 32 birds. Hepatocellular karyomegaly was present in liver tissues from 10 birds (5 birds with typical APV inclusions and 5 birds without discernable inclusions). DNA in situ hybridization recognized intranuclear APV nucleic acid in liver sections of 18 of 32 birds. DNA amplification with Southern or dot blots also identified APV nucleic acid in processed, paraffin-embedded livers of 18 of 32 birds. This study demonstrates that acute APV infection is a frequent cause of multifocal to coalescing hepatocellular necrosis in psittacine birds. Furthermore, APV infection is best diagnosed using DNA probes, especially when typical intranuclear inclusions are not observed microscopically.
对32只患有多灶性至融合性肝细胞坏死的鹦鹉科鸟类的肝脏切片进行检查，以确定病因。禽多瘤病毒（APV）感染（32只鸟中的19只）、细菌性肝炎（32只鸟中的5只）和衣原体病（32只鸟中的3只）是肝脏疾病的主要病因。使用苏木精和伊红（HE）染色、DNA原位杂交以及Southern印迹法进行DNA扩增来证明APV包涵体或核酸的存在。在32只鸟中有5只鸟的HE染色肝脏切片中观察到提示APV感染的嗜双色性核内包涵体。10只鸟的肝脏组织中存在肝细胞核肿大（5只鸟有典型的APV包涵体，5只鸟没有可辨别的包涵体）。DNA原位杂交在32只鸟中的18只鸟的肝脏切片中识别出核内APV核酸。Southern印迹法或斑点印迹法进行的DNA扩增也在32只鸟中的18只鸟的石蜡包埋肝脏组织中鉴定出APV核酸。这项研究表明，急性APV感染是鹦鹉科鸟类多灶性至融合性肝细胞坏死的常见病因。此外，使用DNA探针能最好地诊断APV感染，尤其是在显微镜下未观察到典型核内包涵体时。
