Charles S D, Sreevatsan S, Bey R F, Sivanandan V, Halvorson D A, Nagaraja K V
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
J Vet Diagn Invest. 1996 Jul;8(3):310-4. doi: 10.1177/104063879600800306.
A dot immunobinding assay (DIA) was developed for the detection of antibodies to Salmonella enteritidis. Western blot analysis of outer membrane proteins from SE identified 2 polypeptides of molecular masses 43 and 46 kD that were specific for S. enteritidis. These 2 polypeptides were utilized as antigens in the DIA. The DIA was tested on sera from chickens experimentally infected with S. enteritidis. Results of the DIA were compared with that of conventional microagglutination and serum plate tests. The DIA was a highly specific and sensitive test that can be useful for screening birds to determine if they are infected with S. enteritidis. Its simplicity, reliability, reproducibility, and speed in interpreting the assay results makes it a useful screening test for flock monitoring.
开发了一种斑点免疫结合测定法（DIA）用于检测肠炎沙门氏菌抗体。对肠炎沙门氏菌外膜蛋白的蛋白质印迹分析鉴定出分子量为43和46 kD的2种多肽，它们是肠炎沙门氏菌特有的。这2种多肽被用作DIA中的抗原。用实验性感染肠炎沙门氏菌的鸡的血清对DIA进行检测。将DIA的结果与传统的微量凝集试验和血清平板试验的结果进行比较。DIA是一种高度特异且灵敏的检测方法，可用于筛选禽类以确定它们是否感染了肠炎沙门氏菌。其操作简单、可靠、可重复，且在解释检测结果方面速度快，使其成为用于鸡群监测的有用筛选试验。
