Kim C J, Nagaraja K V, Pomeroy B S
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St Paul 55108.
Am J Vet Res. 1991 Jul;52(7):1069-74.
An ELISA was developed and tested for its ability to detect antibodies against Salmonella enteritidis in chickens. Various features of the ELISA were evaluated and optimized. The outer membrane protein antigens selected by use of the protein immunoblotting method made the assay specific and sensitive. The assay was evaluated in chickens experimentally infected with S enteritidis. Blood samples collected at weekly intervals after experimental infection with S enteritidis were analyzed by ELISA. Results of the ELISA were compared with those of conventional serum plate and microagglutination tests. The ELISA was more sensitive and specific in the detection of S enteritidis infection than the other 2 conventional tests.
开发了一种酶联免疫吸附测定法（ELISA），并对其检测鸡体内抗肠炎沙门氏菌抗体的能力进行了测试。对ELISA的各种特性进行了评估和优化。通过蛋白质免疫印迹法选择的外膜蛋白抗原使该检测方法具有特异性和敏感性。在实验感染肠炎沙门氏菌的鸡身上对该检测方法进行了评估。用ELISA分析了肠炎沙门氏菌实验感染后每周采集的血样。将ELISA的结果与传统血清平板和微量凝集试验的结果进行了比较。在检测肠炎沙门氏菌感染方面，ELISA比其他两种传统检测方法更敏感、更特异。
