Dudar J D
Brain Res. 1977 Jul 1;129(2):237-46. doi: 10.1016/0006-8993(77)90004-x.
Acetylcholine (ACh) was collected from the alvear surface of the dorsal hippocampus and cerebral cortex in chloralose-urethane anaesthetized or unanaesthetized rabbits. With anaesthesia, the resting release of ACh from the hippocampus was greater than that from the cortex. Wthout anaesthesia, the resting release from both areas was much higher and very similar. The addition of atropine sulphate (1 microgram/ml) to the collecting fluid or the administration of Artane (2 mg/kg i.v.) increased resting ACh release from both the hippocampus and cortex to similar output levels. Atropine also increased ACh release due to stimulation of the medial septum (MS) or mesencephalic reticular formation (MRF). Removal of the septum abolished the effect of atropine on resting ACh release and on release evoked by MRF stimulation from both the hippocampus and cortex. The data indicate that the septum is an essential pathway for cholinergic fibres ascending to the cerebral cortex and hippocampus. They also demonstrate that the septal cholinergic fibres must be intact and active for atropine to increase ACh release from their terminals.
在水合氯醛 - 乌拉坦麻醉或未麻醉的家兔中，从背侧海马体和大脑皮层的肺泡表面收集乙酰胆碱（ACh）。麻醉状态下，海马体中ACh的静息释放量大于皮层。未麻醉时，两个区域的静息释放量都高得多且非常相似。向收集液中添加硫酸阿托品（1微克/毫升）或静脉注射安坦（2毫克/千克）可使海马体和皮层的静息ACh释放量增加到相似的水平。阿托品还会因内侧隔区（MS）或中脑网状结构（MRF）的刺激而增加ACh释放。切除隔区消除了阿托品对海马体和皮层静息ACh释放以及MRF刺激诱发的释放的影响。数据表明，隔区是胆碱能纤维上行至大脑皮层和海马体的重要通路。它们还表明，隔区胆碱能纤维必须完整且活跃，阿托品才能增加其终末的ACh释放。
