全氟碳亲和乳液在快速分离金黄色葡萄球菌中的应用。

Applications of perfluorocarbon affinity emulsions for the rapid isolation of Staphylococcus aureus.

作者信息

McCreath G E, Chase H A

机构信息

Department of Chemical Engineering, University of Cambridge, U.K.

出版信息

Biotechnol Prog. 1996 Jan-Feb;12(1):77-83. doi: 10.1021/bp950055i.

DOI:10.1021/bp950055i

PMID:8845111

Abstract

Human immunoglobulin G (IgG) has been immobilized to poly(vinyl alcohol)-stabilized liquid perfluorocarbon droplets. This affinity emulsion has been shown to adsorb Staphylococcus aureus cells from solutions with adsorption capacities of 32 x 10(9) and 48 x 10(9) cells/mL for affinity emulsions with immobilized IgG densities of 1.15 and 2.45 mg/mL, respectively. The kinetics of cell binding from solution were found to be rapid with clearance of S. aureus cells from a suspension (8 x 10(8) cell/mL) achievable in under 5 min. S. aureus cells (1.3 x 10(9) cells) could also be rapidly depleted from a suspension of Saccharomyces cerevisiae (3.4 x 10(10) cell/mL) in under 18 min with no cross-reactivity being observed. The affinity emulsion was stable for at least five cycles of operation with little loss in adsorption capacity when removal of adsorbed cells was carried out at pH 2.5.

摘要

人免疫球蛋白G（IgG）已固定在聚乙烯醇稳定的全氟碳液滴上。这种亲和乳液已被证明能够从溶液中吸附金黄色葡萄球菌细胞，对于固定化IgG密度分别为1.15和2.45mg/mL的亲和乳液，其吸附容量分别为32×10⁹和48×10⁹个细胞/mL。发现溶液中细胞结合的动力学很快，在5分钟内即可从悬浮液（8×10⁸个细胞/mL）中清除金黄色葡萄球菌细胞。在18分钟内，也可以从酿酒酵母悬浮液（3.4×10¹⁰个细胞/mL）中快速去除1.3×10⁹个金黄色葡萄球菌细胞，且未观察到交叉反应。当在pH 2.5下去除吸附的细胞时，亲和乳液在至少五个操作循环中保持稳定，吸附容量几乎没有损失。