Brüstle O, Maskos U, McKay R D
Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892-4092, USA.
Neuron. 1995 Dec;15(6):1275-85. doi: 10.1016/0896-6273(95)90007-1.
The stereotyped positions occupied by individual classes of neurons are a fundamental characteristic of CNS cytoarchitecture. To study the regulation of neuronal positioning, we injected genetically labeled neural precursors derived from dorsal and ventral mouse forebrain into the telencephalic vesicles of embryonic rats. Cells from both areas were found to participate in the generation of telencephalic, diencephalic, and mesencephalic brain regions. Donor-derived neurons populated the host brain in distinct patterns and acquired phenotypic features appropriate for their final location. These observations indicate that neuronal migration and differentiation are predominantly regulated by non-cell-autonomous signals. Exploiting this phenomenon, intrauterine transplantation allows generation of controlled chimerism in the mammalian brain.
各类神经元占据的固定位置是中枢神经系统细胞结构的一个基本特征。为了研究神经元定位的调控机制，我们将源自小鼠前脑背侧和腹侧的基因标记神经前体细胞注射到胚胎大鼠的端脑泡中。发现来自这两个区域的细胞都参与了端脑、间脑和中脑区域的生成。供体来源的神经元以不同模式分布在宿主脑中，并获得了与其最终位置相适应的表型特征。这些观察结果表明，神经元的迁移和分化主要受非细胞自主信号调控。利用这一现象，子宫内移植能够在哺乳动物脑中产生可控的嵌合体。
