Brüstle O, Spiro A C, Karram K, Choudhary K, Okabe S, McKay R D
Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-4092, USA.
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14809-14. doi: 10.1073/pnas.94.26.14809.
During embryogenesis, pluripotent stem cells segregate into daughter lineages of progressively restricted developmental potential. In vitro, this process has been mimicked by the controlled differentiation of embryonic stem cells into neural precursors. To explore the developmental potential of these cell-culture-derived precursors in vivo, we have implanted them into the ventricles of embryonic rats. The transplanted cells formed intraventricular neuroepithelial structures and migrated in large numbers into the brain tissue. Embryonic-stem-cell-derived neurons, astrocytes, and oligodendrocytes incorporated into telencephalic, diencephalic, and mesencephalic regions and assumed phenotypes indistinguishable from neighboring host cells. These observations indicate that entirely in vitro-generated neural precursors are able to respond to environmental signals guiding cell migration and differentiation and have the potential to reconstitute neuronal and glial lineages in the central nervous system.
在胚胎发生过程中,多能干细胞会分离成发育潜能逐渐受限的子谱系。在体外,通过将胚胎干细胞定向分化为神经前体细胞,这一过程已得到模拟。为了在体内探究这些源自细胞培养的前体细胞的发育潜能,我们将它们植入了胚胎大鼠的脑室。移植的细胞形成了脑室内神经上皮结构,并大量迁移到脑组织中。源自胚胎干细胞的神经元、星形胶质细胞和少突胶质细胞整合到端脑、间脑和中脑区域,并呈现出与相邻宿主细胞难以区分的表型。这些观察结果表明,完全在体外生成的神经前体细胞能够对引导细胞迁移和分化的环境信号做出反应,并具有在中枢神经系统中重构神经元和神经胶质谱系的潜力。