Oligodendrocyte precursors originate from both the dorsal and the ventral parts of the spinal cord.

Oligodendrocytes have been recently claimed to originate from bilateral columns of precursors whose extension is limited to the ventral region of the neuroepithelium. We designed an experiment in which the developmental capabilities of the different regions of the ventricular epithelium could be tested. We exchanged isotopically and isochronically defined sectors of the E2 spinal cord between quail and chick embryos and followed the production and migration of oligodendrocytes by using a quail-specific cDNA probe encoding the oligodendrocyte marker Schwann cell myelin protein. We showed that oligodendrocytes are generated in vivo from both ventral and dorsal halves of the neural tube. Moreover, extensive ventrodorsal, as well as dorsoventral, migrations of oligodendrogenic cells take place during spinal cord differentiation.