Cameron-Curry P, Le Douarin N M
Institut d'Embryologie cellulaire et moléculaire, CNRS, Nogent-sur-Marne, France.
Neuron. 1995 Dec;15(6):1299-310. doi: 10.1016/0896-6273(95)90009-8.
Oligodendrocytes have been recently claimed to originate from bilateral columns of precursors whose extension is limited to the ventral region of the neuroepithelium. We designed an experiment in which the developmental capabilities of the different regions of the ventricular epithelium could be tested. We exchanged isotopically and isochronically defined sectors of the E2 spinal cord between quail and chick embryos and followed the production and migration of oligodendrocytes by using a quail-specific cDNA probe encoding the oligodendrocyte marker Schwann cell myelin protein. We showed that oligodendrocytes are generated in vivo from both ventral and dorsal halves of the neural tube. Moreover, extensive ventrodorsal, as well as dorsoventral, migrations of oligodendrogenic cells take place during spinal cord differentiation.
最近有观点认为，少突胶质细胞起源于双侧的前体细胞柱，其延伸仅限于神经上皮的腹侧区域。我们设计了一项实验，可对脑室上皮不同区域的发育能力进行测试。我们在鹌鹑和鸡胚胎之间，对E2期脊髓的同位素标记且时间相同的区域进行了交换，并使用编码少突胶质细胞标记物施万细胞髓磷脂蛋白的鹌鹑特异性cDNA探针，追踪少突胶质细胞的产生和迁移。我们发现，少突胶质细胞在体内由神经管的腹侧和背侧两半区域产生。此外，在脊髓分化过程中，少突胶质细胞发生了广泛的腹背向以及背腹向迁移。
