Christopoulos G, Paxinos G, Huang X F, Beaumont K, Toga A W, Sexton P M
St. Vincent's Institute of Medical Research, Victoria, Australia.
Can J Physiol Pharmacol. 1995 Jul;73(7):1037-41. doi: 10.1139/y95-146.
The distribution of amylin receptors (125I-labelled rat amylin) in brains of rat and monkey were mapped and compared with the distribution of receptors for calcitonin (CT) (125I-labelled salmon CT) and calcitonin gene related peptide (CGRP) (rat, 125I-labelled rat CGRP alpha; monkey, 125I-labelled human CGRP alpha. In rat, amylin receptors were discretely distributed with the highest receptor densities found in mid-caudal accumbens nucleus, parts of the bed nucleus of the stria terminalis, amygdala, and hypothalamus. Moderate to high densities of binding also occurred in the area postrema, subfornical organ, vascular organ of the lamina terminalis, locus ceruleus, dorsal raphe, and caudal solitary tract nucleus. In monkey, the distribution of amylin binding sites was similar, although the highest densities of receptors were in the hypothalamus, with relatively fewer sites present in the accumbens nucleus. In rat, the distribution of amylin receptors formed a subset of the receptor distributions for 125I-labelled salmon CT and 125I-labelled rat CGRP alpha. In contrast, in monkey, although the amylin receptors again formed a subset of the binding sites identified with 125I-labelled salmon CT, there was very little overlap with the pattern of CGRP receptor distribution. This suggests that the specificity profile of amylin receptors in primates differs from that of amylin receptors in the rat, with CGRP alpha having relatively lower affinity for the primate amylin receptors.
绘制了大鼠和猴脑中胰岛淀粉样多肽受体（¹²⁵I标记的大鼠胰岛淀粉样多肽）的分布图，并与降钙素（CT）受体（¹²⁵I标记的鲑鱼降钙素）和降钙素基因相关肽（CGRP）受体（大鼠，¹²⁵I标记的大鼠CGRPα；猴，¹²⁵I标记的人CGRPα）的分布图进行了比较。在大鼠中，胰岛淀粉样多肽受体呈离散分布，在尾中部伏隔核、终纹床核的部分区域、杏仁核和下丘脑发现受体密度最高。在最后区、穹窿下器官、终板血管器、蓝斑、中缝背核和尾侧孤束核也出现中度至高结合密度。在猴中，胰岛淀粉样多肽结合位点的分布相似，尽管受体密度最高的区域是下丘脑，伏隔核中的位点相对较少。在大鼠中，胰岛淀粉样多肽受体的分布形成了¹²⁵I标记的鲑鱼降钙素和¹²⁵I标记的大鼠CGRPα受体分布的一个子集。相反，在猴中，尽管胰岛淀粉样多肽受体再次形成了¹²⁵I标记的鲑鱼降钙素所识别的结合位点的一个子集，但与CGRP受体分布模式的重叠非常少。这表明灵长类动物中胰岛淀粉样多肽受体的特异性谱与大鼠中胰岛淀粉样多肽受体的不同，CGRPα对灵长类动物胰岛淀粉样多肽受体的亲和力相对较低。
