Sexton P M, Paxinos G, Kenney M A, Wookey P J, Beaumont K
St Vincent's Institute of Medical Research, Fitzroy, Victoria, Australia.
Neuroscience. 1994 Sep;62(2):553-67. doi: 10.1016/0306-4522(94)90388-3.
Amylin is a recently discovered 37 amino acid peptide which is co-secreted from the pancreas with insulin and acts to modulate carbohydrate metabolism. Recently, high-affinity binding sites for [125I]rat amylin have been identified in the rat central nervous system. These sites also have high affinity for the structurally related peptides calcitonin gene-related peptide and salmon calcitonin. In the present study we have used in vitro autoradiography to map the distribution of these [125I]rat amylin binding sites in rat brain. High to moderate levels of binding were present in mid-caudal accumbens nucleus, fundus striati and parts of the bed nucleus of the stria terminalis and substantia inominata. This binding extended caudally into parts of the amygdalostriatal transition zone and the central and medial amygdaloid nuclei. High to moderate levels of binding also occurred in much of the hypothalamus including the medial preoptic, dorsomedial hypothalamic and medial tuberal nuclei as well as the ventrolateral subnucleus of the ventromedial hypothalamic nucleus. Other regions of high level binding included the subfornical organ, the vascular organ of the lamina terminalis, area postrema, locus coeruleus, dorsal raphe and caudal parts of the nucleus of the solitary tract. The subfornical organ, vascular organ of the lamina terminalis and area postrema, which display some of the highest binding densities, lack a patent blood-brain barrier and thus could be responsive to blood-borne amylin. In conclusion we have mapped, in detail, the distribution of amylin binding sites in rat brain. The location of binding is consistent with potential roles for these sites in appetite, fluid and electrolyte homeostasis, autonomic function and regulation of mood.
胰淀素是一种最近发现的由37个氨基酸组成的肽，它与胰岛素一起从胰腺共同分泌，作用是调节碳水化合物代谢。最近，在大鼠中枢神经系统中已鉴定出[125I]大鼠胰淀素的高亲和力结合位点。这些位点对结构相关的肽降钙素基因相关肽和鲑鱼降钙素也具有高亲和力。在本研究中，我们使用体外放射自显影技术来绘制这些[125I]大鼠胰淀素结合位点在大鼠脑中的分布。在中尾伏隔核、纹状体底部以及终纹床核和无名质的部分区域存在高到中等水平的结合。这种结合向尾侧延伸到杏仁核纹状体过渡区的部分以及中央和内侧杏仁核。在包括内侧视前核、下丘脑背内侧核和内侧结节核以及腹内侧下丘脑核的腹外侧亚核在内的大部分下丘脑区域也出现高到中等水平的结合。其他高水平结合区域包括穹窿下器官、终板血管器、最后区、蓝斑、中缝背核和孤束核的尾部。穹窿下器官、终板血管器和最后区显示出一些最高的结合密度，它们缺乏完整的血脑屏障，因此可能对血源性胰淀素有反应。总之，我们详细绘制了大鼠脑中胰淀素结合位点的分布。结合位点的位置与这些位点在食欲、液体和电解质稳态、自主功能和情绪调节中的潜在作用一致。
