Millar J C, Kaufman P L
Department of Ophthalmology & Visual Sciences, University of Wisconsin-Madison 53792-3220, USA.
Exp Eye Res. 1995 Dec;61(6):677-83. doi: 10.1016/s0014-4835(05)80018-1.
We investigated whether (i) single or repeated topical treatment with either the tromethamine salt or the isopropyl ester of prostaglandin F2 alpha (PGF2 alpha-TS or PGF2 alpha-IE) inhibits the accommodative response to pilocarpine; (ii) pilocarpine given after PGF2 alpha inhibits the latter's ocular hypotensive effect, as occurs with the reverse order of administration. PGF2 alpha-IE (2 micrograms) or PGF2 alpha-TS (50 micrograms) was applied topically to the right eye of five cynomolgus monkeys twice daily for 4 days. Before initial dosing, and 1 hr after the initial and final dose the animals were anesthetized with ketamine, and intraocular pressure (IOP) and refraction determined bilaterally. Following post-PG IOP and refraction determinations, pilocarpine HCl was given topically (bilaterally) or infused i.m. over 10 min, after which refraction was determined periodically for 50 min. IOP was then again determined. After a single unilateral dose of PGF2 alpha-TS or PGF2 alpha-IE, IOP was unchanged. Both eyes accommodated markedly and equally in response to pilocarpine, and IOP fell bilaterally by a statistically significant 3 mmHg in response to pilocarpine. Repeated doses of PGF2 alpha-TS or PGF2 alpha-IE did not significantly affect the accommodative response to pilocarpine, while pilocarpine did not alter the approximately 10 mmHg ipsilateral IOP fall induced by repeated dosing of PGF2 alpha-TS or PGF2 alpha-IE. It is concluded that repeated unilateral topical application of PGF2 alpha-TS or PGF2 alpha-IE to the cynomolgus monkey eye markedly reduces ipsilateral IOP, but has no effect on the ipsilateral or contralateral eye accommodative response to pilocarpine. Pilocarpine reverses the PGF2 alpha-TS or PGF2 alpha-IE-induced fall in IOP when given before the PGF2 alpha, but does not prevent it when given after, within the parameters of this study.
（i）用前列腺素F2α的 tromethamine 盐或异丙酯（PGF2α-TS 或 PGF2α-IE）进行单次或重复局部治疗是否会抑制对毛果芸香碱的调节反应；（ii）如给药顺序相反时那样，在 PGF2α给药后给予毛果芸香碱是否会抑制后者的降眼压作用。将 PGF2α-IE（2 微克）或 PGF2α-TS（50 微克）每天两次局部应用于 5 只食蟹猴的右眼，持续 4 天。在首次给药前，以及首次和末次给药后 1 小时，用氯胺酮麻醉动物，并双侧测定眼压（IOP）和屈光度。在 PG 后测定 IOP 和屈光度后，局部（双侧）给予盐酸毛果芸香碱或在 10 分钟内静脉注射，之后每隔 50 分钟定期测定屈光度。然后再次测定 IOP。单次单侧给予 PGF2α-TS 或 PGF2α-IE 后，IOP 无变化。双眼对毛果芸香碱的反应均明显且同等程度地调节，并且对毛果芸香碱的反应使双侧 IOP 下降了具有统计学意义的 3 mmHg。重复给予 PGF2α-TS 或 PGF2α-IE 对毛果芸香碱的调节反应没有显著影响，而毛果芸香碱并未改变重复给予 PGF2α-TS 或 PGF2α-IE 所引起的同侧约 10 mmHg 的 IOP 下降。得出的结论是，在食蟹猴眼中重复单侧局部应用 PGF2α-TS 或 PGF2α-IE 可显著降低同侧 IOP，但对同侧或对侧眼对毛果芸香碱的调节反应没有影响。在本研究的参数范围内，毛果芸香碱在 PGF2α之前给药时可逆转 PGF2α-TS 或 PGF2α-IE 引起的 IOP 下降，但在 PGF2α之后给药时则不能阻止这种下降。
